Toxigenic and Non-Toxigenic Patterns I, Ii and Iii and Biofilm-Forming Ability in Bacteroides Fragilis Strains Isolated From Patients Diagnosed With Colorectal Cancer

Toxigenic and Non-Toxigenic Patterns I, Ii and Iii and Biofilm-Forming Ability in Bacteroides Fragilis Strains Isolated From Patients Diagnosed With Colorectal Cancer Publisher

Jasemi S¹ ; Emaneini M¹ ; Fazeli MS² ; Ahmadinejad Z³ ; Nomanpour B⁴ ; Sadeghpour Heravi F⁵ ; Sechi LA⁶ ; Feizabadi MM^{1, 6}

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1. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Surgery, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Infectious Diseases, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran
4. Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
5. Surgical Infection Research Group, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, Australia
6. Department of Biomedical Sciences, University of Sassari, Sassari, Italy

Source: Gut Pathogens Published:2020

Abstract

Background: Enterotoxigenic Bacteroides fragilis (ETBF) associated with the initiation and progression of colorectal cancer (CRC) has been alarmingly reported all over the world. In this study, simultaneous investigation of toxigenic and non-toxigenic patterns I, II and III and biofilm formation ability of Bacteroides fragilis isolated from patients with colorectal cancer was performed. Methods: Thirty-one patients diagnosed with CRC and thirty-one control subjects were recruited in this study. Specimens were cultured on BBE and BBA culture media. Classical phenotypic identification tests and PCR was performed to verify Bacteroides fragilis presence. Also, biofilm-forming ability and expression of bft gene were assessed under biofilm and planktonic forms. Results: A total of 68 B.fragilis was isolated from all colorectal tissue, of which 13 isolates (19.1%) (11 isolates from CRC and 2 from normal tissue) were positive for bft gene. The abundance patterns of I, II and III were as follow in descending order; pattern I > pattern III > pattern II in CRC subjects and pattern II > pattern III > pattern I in normal tissues. Also, pattern I showed higher biofilm formation ability compared to other patterns. Toxin expression was significantly reduced in biofilm form comparing with planktonic form. Conclusions: Based on our findings, there was a difference between the abundance of patterns I, II, and III and biofilm formation in isolates obtained from CRC and normal tissues. Biofilm formation ability and toxin encoding gene (bft) are two main virulence factors in B. fragilis pathogenicity which require more investigation to treat B. fragilis infections effectively. © 2020 The Author(s).

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Mohammad Mehdi Feizabadi (3)

Masoumeh Douraghi (1)

Style	Citing Format
MLA	Jasemi S, et al.. "Toxigenic and Non-Toxigenic Patterns I, Ii and Iii and Biofilm-Forming Ability in Bacteroides Fragilis Strains Isolated From Patients Diagnosed With Colorectal Cancer." Gut Pathogens, vol. 12, no. 1, 2020, pp. -.
APA	Jasemi S, Emaneini M, Fazeli MS, Ahmadinejad Z, Nomanpour B, Sadeghpour Heravi F, Sechi LA, Feizabadi MM (2020). Toxigenic and Non-Toxigenic Patterns I, Ii and Iii and Biofilm-Forming Ability in Bacteroides Fragilis Strains Isolated From Patients Diagnosed With Colorectal Cancer. Gut Pathogens, 12(1), -.
Chicago	Jasemi S, Emaneini M, Fazeli MS, Ahmadinejad Z, Nomanpour B, Sadeghpour Heravi F, Sechi LA, Feizabadi MM. "Toxigenic and Non-Toxigenic Patterns I, Ii and Iii and Biofilm-Forming Ability in Bacteroides Fragilis Strains Isolated From Patients Diagnosed With Colorectal Cancer." Gut Pathogens 12, no. 1 (2020): -.
Harvard	Jasemi S et al. (2020) 'Toxigenic and Non-Toxigenic Patterns I, Ii and Iii and Biofilm-Forming Ability in Bacteroides Fragilis Strains Isolated From Patients Diagnosed With Colorectal Cancer', Gut Pathogens, 12(1), pp. -.
Vancouver	Jasemi S, Emaneini M, Fazeli MS, Ahmadinejad Z, Nomanpour B, Sadeghpour Heravi F, et al.. Toxigenic and Non-Toxigenic Patterns I, Ii and Iii and Biofilm-Forming Ability in Bacteroides Fragilis Strains Isolated From Patients Diagnosed With Colorectal Cancer. Gut Pathogens. 2020;12(1):-.
BibTex	@article{ author = {Jasemi S and Emaneini M and Fazeli MS and Ahmadinejad Z and Nomanpour B and Sadeghpour Heravi F and Sechi LA and Feizabadi MM}, title = {Toxigenic and Non-Toxigenic Patterns I, Ii and Iii and Biofilm-Forming Ability in Bacteroides Fragilis Strains Isolated From Patients Diagnosed With Colorectal Cancer}, journal = {Gut Pathogens}, volume = {12}, number = {1}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Jasemi S AU - Emaneini M AU - Fazeli MS AU - Ahmadinejad Z AU - Nomanpour B AU - Sadeghpour Heravi F AU - Sechi LA AU - Feizabadi MM TI - Toxigenic and Non-Toxigenic Patterns I, Ii and Iii and Biofilm-Forming Ability in Bacteroides Fragilis Strains Isolated From Patients Diagnosed With Colorectal Cancer JO - Gut Pathogens VL - 12 IS - 1 SP - EP - PY - 2020 ER -

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