Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53

Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53 Publisher

Rajabi Pour M^{1, 2, 4} ; Fardid R^{1, 3, 4} ; Zare T⁵ ; Kargar Shouroki F⁶ ; Moslehshirazi MA^{1, 3, 4} ; Behzad Behbahani A⁷

Source: Journal of Biomedical Physics and Engineering Published:2020

Abstract

Background: Some operating room personnel are occupationally exposed to genotoxic agents such as anesthetic gases and ionizing radiation. Adaptive response, as a defense mechanism, will occur when cells become exposed to a low dose of factors harming DNA (priming dose), which in the subsequent exposure to higher dose of those factors (challenging dose), show more resistance and sensibility. Objective: The aim of this study was to investigate adaptive response or synergy of ionizing radiation in the operating room personnel exposed to anesthetic gases by evaluation of the relative gene expression changes of effective genes for DNA repair such as Ku80, Ligase1 and P53. Material and Methods: In this case-control study, 20 operating room personnel and 20 nurses (who were not present in the operating room) as controls were studied. Venous blood samples were drawn from participants. In order to evaluate the adaptive response, a challenging dose of 2Gy gamma radiation was applied to blood samples. Moreover, RNA extraction and cDNA synthesis were performed. Gene expression level was studied by RT-qPCR and compared with the control group. Results: Ligase1 and P53 expression in the operating room personnel was signifi-cantly higher than that of the control group before irradiation (P˂0.001). Statistically, there was no significant difference in the Ku80 and P53 expression in the operating room personnel before and after irradiation. Conclusion: Given the findings of this study, exposure to challenging dose of gamma radiation can induce adaptive response in expression of Ku80 and P53 genes in operating room personnel. © 2020, Shiraz University of Medical Sciences. All rights reserved.

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Style	Citing Format
MLA	Rajabi Pour M, et al.. "Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53." Journal of Biomedical Physics and Engineering, vol. 10, no. 2, 2020, pp. 225-234.
APA	Rajabi Pour M, Fardid R, Zare T, Kargar Shouroki F, Moslehshirazi MA, Behzad Behbahani A (2020). Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53. Journal of Biomedical Physics and Engineering, 10(2), 225-234.
Chicago	Rajabi Pour M, Fardid R, Zare T, Kargar Shouroki F, Moslehshirazi MA, Behzad Behbahani A. "Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53." Journal of Biomedical Physics and Engineering 10, no. 2 (2020): 225-234.
Harvard	Rajabi Pour M et al. (2020) 'Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53', Journal of Biomedical Physics and Engineering, 10(2), pp. 225-234.
Vancouver	Rajabi Pour M, Fardid R, Zare T, Kargar Shouroki F, Moslehshirazi MA, Behzad Behbahani A. Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53. Journal of Biomedical Physics and Engineering. 2020;10(2):225-234.
BibTex	@article{ author = {Rajabi Pour M and Fardid R and Zare T and Kargar Shouroki F and Moslehshirazi MA and Behzad Behbahani A}, title = {Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53}, journal = {Journal of Biomedical Physics and Engineering}, volume = {10}, number = {2}, pages = {225-234}, year = {2020} }
RIS	TY - JOUR AU - Rajabi Pour M AU - Fardid R AU - Zare T AU - Kargar Shouroki F AU - Moslehshirazi MA AU - Behzad Behbahani A TI - Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53 JO - Journal of Biomedical Physics and Engineering VL - 10 IS - 2 SP - 225 EP - 234 PY - 2020 ER -

Style

Citing Format

MLA

Rajabi Pour M, et al.. "Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53." Journal of Biomedical Physics and Engineering, vol. 10, no. 2, 2020, pp. 225-234.

APA

Rajabi Pour M, Fardid R, Zare T, Kargar Shouroki F, Moslehshirazi MA, Behzad Behbahani A (2020). Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53. Journal of Biomedical Physics and Engineering, 10(2), 225-234.

Chicago

Rajabi Pour M, Fardid R, Zare T, Kargar Shouroki F, Moslehshirazi MA, Behzad Behbahani A. "Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53." Journal of Biomedical Physics and Engineering 10, no. 2 (2020): 225-234.

Harvard

Rajabi Pour M et al. (2020) 'Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53', Journal of Biomedical Physics and Engineering, 10(2), pp. 225-234.

Vancouver

Rajabi Pour M, Fardid R, Zare T, Kargar Shouroki F, Moslehshirazi MA, Behzad Behbahani A. Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53. Journal of Biomedical Physics and Engineering. 2020;10(2):225-234.

BibTex

@article{ author = {Rajabi Pour M and Fardid R and Zare T and Kargar Shouroki F and Moslehshirazi MA and Behzad Behbahani A}, title = {Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53}, journal = {Journal of Biomedical Physics and Engineering}, volume = {10}, number = {2}, pages = {225-234}, year = {2020} }

RIS

TY - JOUR
AU - Rajabi Pour M
AU - Fardid R
AU - Zare T
AU - Kargar Shouroki F
AU - Moslehshirazi MA
AU - Behzad Behbahani A
TI - Assessment of Adaptive Response of Gamma Radiation in the Operating Room Personnel Exposed to Anesthetic Gases by Measuring the Relative Gene Expression Changes Ku80, Ligase1 and P53
JO - Journal of Biomedical Physics and Engineering
VL - 10
IS - 2
SP - 225
EP - 234
PY - 2020
ER -

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