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Investigating the Expression Level of Nf-Kb and Hif1a Genes Among the Inhabitants of Two Different Background Radiation Areas in Ramsar, Iran Publisher Pubmed



Talebian H1, 5 ; Monfared AS3 ; Niaki HA4 ; Fattahi S5, 6 ; Bakhtiari E5 ; Changizi V2
Authors
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Authors Affiliations
  1. 1. Student Research Committee, Tehran University of Medical Sciences, Tehran, IR, Iran
  2. 2. Department of Technology of Radiology and Radiotherapy, Alliend Medical Sciences School, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Cancer Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, IR, Iran
  4. 4. Department of Genetics, School of Medicine, Babol University of Medical Sciences, Babol, IR, Iran
  5. 5. Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences Babol, IR, Iran
  6. 6. North Research Centre of Pasteur Institute, Amol, IR, Iran

Source: Journal of Environmental Radioactivity Published:2020


Abstract

This study investigated the fluctuation of NF-KB and HIF-1a gene expression between inhabitants of a high-level background radiation area (HBRA) and a normal-level background radiation area (NBRA) of Ramsar, Iran. Sixty participants with the mean age of 48 ± 15 years were selected and divided into two groups. The group receiving a dose of ≤1.5 mGy/year (NBRA) was considered the control group and the target group (HBRA) received a dose of >1.5 mGy/year. These two groups were from neighbor regions to minimize socioeconomic differences between the participants. Blood samples were collected from each group and NF-KB and HIF-1a expression levels were compared using quantitative real-time PCR (qPCR) based on the stem loop method. The effects of residency duration in the respective areas and gender on the expression of NF-KB and HIF-1a was also examined. The HIF-1a expression level was statistically lower in the HLBRA region (P < 0.0002), while NF-KB expression was upregulated (P < 0.0001). Although the under-expression of HIF-1a in response to dose rate was significant in females (P < 0.0004), it was not different in males (P = 0.74), indicating a significant difference between sexes (P = 0.0047). The upregulation of NF-KB expression related to dose level was also significant for the female group (P < 0.0001), whereas it was not for the male group (P = 0.72). Notably and as expected, there was a significant relation between longer residency in the HBRA and HIF-1A under-expression (P < 0.026), while there was no effect of increasing residency time for NF-KB over-expression level (P = 0.29). The dwellers of the HBRA those noted that despite receiving an elevated radiation level were seemingly good in general health, showed some alterations in their molecular mechanisms, specifically HIF-1a and NF-KB expression levels. It is not clear if this is indicative of a beneficial adaptive response and more research is recommended. © 2020 Elsevier Ltd
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