Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Effect of Heparin on Bone Metabolism and Orthodontic Tooth Movement in Rats Publisher Pubmed



Salari B1 ; Moradian R2 ; Kheirandish Y3, 4 ; Alaeddini M4 ; Etemadmoghadam S4 ; Maghsoudi S1 ; Dehpour AR5, 6
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Orthodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  2. 2. Student Research Committee, School of Dentistry, Aja University of Medical Sciences, Tehran, Iran
  3. 3. Department of Dentomaxillofacial Radiology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Clinical and Experimental Dental Research Published:2025


Abstract

Objectives: Various attempts have been made to increase the rate of orthodontic tooth movement (OTM). The aim of this study was to determine the effect of different doses of heparin on OTM and paraclinical factors related to bone metabolism in rats. Methods and Materials: A total of 24 Sprague-Dawley rats were randomly divided into three groups of 8 animals each and injected with 0 (control), 3000, and 6000 U/Kg/d heparin sulfate for 4 weeks. Radiographs were obtained at the initiation and at the end of the study period. Orthodontic forces were applied on Day 14 and continued for the next 2 weeks, after which, OTM, optical density, parathyroid hormone (PTH) level, and histologic variables were assessed for each rat. The latter was performed on hematoxylin/eosin–stained sections of the mesial roots of the first molar and included calculation of the osteoclast number, and resorption lacunae depth and number. One-way analysis of variance, the Tukey test, and a paired-t-test were used for statistical analysis (p < 0.05). Results: A significant increase in OTM, the number of resorptive lacunae, and PTH secretion was observed in the group that received 6000 U/Kg/d compared with both the other groups. There was no significant difference in optical density, and, therefore, bone density, among the study groups (p > 0.05). Conclusion: Heparin injection affects bone metabolism in rats, as shown by the increases in OTM and PTH and its impact on histologic parameters. These effects seem to be dose-dependent and may be a factor that should be taken into consideration during orthodontic treatment planning. © 2024 The Author(s). Clinical and Experimental Dental Research published by John Wiley & Sons Ltd.
Related Docs
1. Korean Panax Ginseng Reduces Orthodontic Tooth Movement in Rats, Applied Sciences (Switzerland) (2021)