Molecular Epidemiology and Antifungal Susceptibility Profiles of Clinical Cryptococcus Neoformans/Cryptococcus Gattii Species Complex

Molecular Epidemiology and Antifungal Susceptibility Profiles of Clinical Cryptococcus Neoformans/Cryptococcus Gattii Species Complex Publisher Pubmed

Bandalizadeh Z¹ ; Shokohi T^{2, 3} ; Badali H^{2, 3} ; Abastabar M^{2, 3} ; Babamahmoudi F⁴ ; Davoodi L⁴ ; Mardani M⁵ ; Javanian M⁶ ; Cheraghmakani H⁷ ; Sepidgar AA⁸ ; Badiee P⁹ ; Khodavaisy S¹⁰ ; Afshari SAK¹¹ ; Ahmadikia K¹⁰ Show All Authors

Bandalizadeh Z¹
Shokohi T^{2, 3}
Badali H^{2, 3}
Abastabar M^{2, 3}
Babamahmoudi F⁴
Davoodi L⁴
Mardani M⁵
Javanian M⁶
Cheraghmakani H⁷
Sepidgar AA⁸
Badiee P⁹
Khodavaisy S¹⁰
Afshari SAK¹¹
Ahmadikia K¹⁰
Seyedmousavi S^{3, 12, 13}

Show Affiliations

1. Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran
2. Department of Medical Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
3. Invasive Fungi Research Center, Mazandaran University of Medical Sciences, Sari, Iran
4. Antimicrobial Resistance Research Center, Mazandaran University of Medical Sciences, Sari, Iran
5. Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6. Infectious Diseases and Tropical Medicine Center, Health Research Institue, Babol University of Medical Sciences, Babol, Iran
7. Department of Neurology, Bu-Ali Hospital, Mazandaran University of Medical Sciences, Sari, Iran
8. Department of Medical Parasitology and Mycology, Babol University of Medical Sciences, Babol, Iran
9. Alborzi Clinical Microbiology Research Center, Shiraz university of Medical Sciences, Shiraz, Iran
10. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
11. Department of Medical Mycology and Parasitology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
12. Center of Expertise in Microbiology, Infection Biology, and Antimicrobial Pharmacology, Tehran, Iran
13. Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, United States

Source: Journal of Medical Microbiology Published:2020

Abstract

Introduction. Limited data regarding the epidemiology and susceptibility profiles of cryptococcosis are available in the Middle East. Aim. Our study aimed to evaluate the molecular diversity, mating types and antifungal susceptibility pattern of Cryptococcus species (n=14) isolated from 320 suspected patients with cryptococcosis. Methodology. The URA5 gene was subjected to restriction fragment length polymorphism and sequence analysis. In addition, in vitro antifungal susceptibility testing was performed by Clinical and Laboratory Standards Institute (CLSI) M27-A4 and M59 guidelines. Results. Overall, 14 (4.4%) patients were confirmed as cryptococcosis. Based on molecular type, 85.7 and 14.3% of the isolates were C. neoformans VN I and VN II, respectively. Phylogenetic analysis of URA5 gene sequences revealed clustering of VN I and VN II isolates into two distinct clades with a substantial difference within each molecular type. Voriconazole and 5-fluorocytosine, respectively, had the lowest (0.031 μg ml−1) and highest (8 μg ml−1) MICs. The epidemiological cutoff values (ECVs) for amphotericin B, fluconazole, voriconazole and 5-fluorocytosine encompassed ≥97% of all 14 C. neoformans VN I species. However, according to the CLSI document M59, ECVs for itraconazole (7; 50% of the isolates) and for posaconazole (1; 7.1% of the isolate), were one log2 dilution higher than the wild type range. Combinations of amphotericin B with 5-fluorocytosine, amphotericin B with fluconazole and fluconazole with 5-fluorocytosine exhibited synergistic effects against 37, 31 and 12.5% of the isolates, respectively. Conclusion. Our findings may significantly contribute to the development of management strategies for patients at a higher risk of cryptococcosis, particularly HIV-positive individuals. © 2020 The Authors

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Style	Citing Format
MLA	Bandalizadeh Z, et al.. "Molecular Epidemiology and Antifungal Susceptibility Profiles of Clinical Cryptococcus Neoformans/Cryptococcus Gattii Species Complex." Journal of Medical Microbiology, vol. 69, no. 1, 2020, pp. 72-81.
APA	Bandalizadeh Z, Shokohi T, Badali H, Abastabar M, Babamahmoudi F, Davoodi L, Mardani M, Javanian M, Cheraghmakani H, Sepidgar AA, Badiee P, Khodavaisy S, Afshari SAK, Ahmadikia K, Seyedmousavi S (2020). Molecular Epidemiology and Antifungal Susceptibility Profiles of Clinical Cryptococcus Neoformans/Cryptococcus Gattii Species Complex. Journal of Medical Microbiology, 69(1), 72-81.
Chicago	Bandalizadeh Z, Shokohi T, Badali H, Abastabar M, Babamahmoudi F, Davoodi L, Mardani M, et al.. "Molecular Epidemiology and Antifungal Susceptibility Profiles of Clinical Cryptococcus Neoformans/Cryptococcus Gattii Species Complex." Journal of Medical Microbiology 69, no. 1 (2020): 72-81.
Harvard	Bandalizadeh Z et al. (2020) 'Molecular Epidemiology and Antifungal Susceptibility Profiles of Clinical Cryptococcus Neoformans/Cryptococcus Gattii Species Complex', Journal of Medical Microbiology, 69(1), pp. 72-81.
Vancouver	Bandalizadeh Z, Shokohi T, Badali H, Abastabar M, Babamahmoudi F, Davoodi L, et al.. Molecular Epidemiology and Antifungal Susceptibility Profiles of Clinical Cryptococcus Neoformans/Cryptococcus Gattii Species Complex. Journal of Medical Microbiology. 2020;69(1):72-81.
BibTex	@article{ author = {Bandalizadeh Z and Shokohi T and Badali H and Abastabar M and Babamahmoudi F and Davoodi L and Mardani M and Javanian M and Cheraghmakani H and Sepidgar AA and Badiee P and Khodavaisy S and Afshari SAK and Ahmadikia K and Seyedmousavi S}, title = {Molecular Epidemiology and Antifungal Susceptibility Profiles of Clinical Cryptococcus Neoformans/Cryptococcus Gattii Species Complex}, journal = {Journal of Medical Microbiology}, volume = {69}, number = {1}, pages = {72-81}, year = {2020} }
RIS	TY - JOUR AU - Bandalizadeh Z AU - Shokohi T AU - Badali H AU - Abastabar M AU - Babamahmoudi F AU - Davoodi L AU - Mardani M AU - Javanian M AU - Cheraghmakani H AU - Sepidgar AA AU - Badiee P AU - Khodavaisy S AU - Afshari SAK AU - Ahmadikia K AU - Seyedmousavi S TI - Molecular Epidemiology and Antifungal Susceptibility Profiles of Clinical Cryptococcus Neoformans/Cryptococcus Gattii Species Complex JO - Journal of Medical Microbiology VL - 69 IS - 1 SP - 72 EP - 81 PY - 2020 ER -

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