Antibacterial Activity of Gold Nanoparticles Conjugated by Aminoglycosides Against A. Baumannii Isolates From Burn Patients

Antibacterial Activity of Gold Nanoparticles Conjugated by Aminoglycosides Against A. Baumannii Isolates From Burn Patients Publisher Pubmed

Rad MR¹ ; Kazemian H² ; Yazdani F³ ; Monfared MRZ⁴ ; Rahdar H¹ ; Javadi A⁵ ; Kodori M⁶

Show Affiliations

1. Department of Medical microbiology, School of Medicine, Tehran University of Medical Science, Tehran, Iran
2. Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran
3. Molecular Microbiology Research Center, Faculty of Medicine, Shahed University, Tehran, Iran
4. Qom Branch Islamic Azad University, Qom, Iran
5. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
6. Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Recent Patents on Anti-Infective Drug Discovery Published:2018

Abstract

Background and Objective: Today, resistance to multiple classes of antibiotics, and notably to the β-lactam and aminoglycosides in A. baumannii is becoming a great problem and it necessitates to make a new approach to combat with multidrug-resistant (MDR), extensive drug-resistance (XDR) or Pandrug-resistant (PDR) isolates. In this case, a new strategy and ways should be designed and introduced against such infections. Therefore the aim of the present study was the evaluation of antibacterial activity of nanoconjugate gentamicin and amikacin with gold against clinical isolates of A. baumannii that were collected from burn wound infection. There are some patents of gold nanoparticles that are conjugated with antibiotics (WO2017161296A1, US20090181101A1). Methods: Eighteen A. baumannii were collected from burn wound infections. For confirmation and detection of aminoglycoside-resistant genes, PCR was carried out. Gold nanoparticles and nanoconjugates were prepared according to the protocol. For the evaluation of the nanoconjugate, Dynamic light cattering, Transmission electron microscopy and FTIR Analysis were carried out. Then, the antibacterial activity of nanoconjugates was conducted by using micro broth dilution method. Result: Prevalence of aminoglycoside-resistant genes was aacC1, aphA6, aadA1, aadB genes 55.5%, 22.2%, 38.8% and 22.2% respectively. Synthesis of bare nanoconjugates resulted in nanoparticle in a size of 10 nm. Amikacin bound to Gnps showed excellent antibacterial activity (94.5%) and just one isolate showed intermediate resistance. Also, gentamicin bound to Gnps had a good antimicrobial effect (50%) in contrast to gentamicin alone. Conclusion: Our study showed that a combination of amikacin and gentamicin with Gnps has a significant antibacterial efficiency against clinical isolates of A. baumannii. Gnps can be used as extraordinary molecular carriers for targeting, and delivery of the antibiotic molecules to the specific infection. © 2018 Bentham Science Publishers.

2. Diversity of Aminoglycoside Modifying Enzymes and 16S Rrna Methylases in Acinetobacter Baumannii and Acinetobacter Nosocomialis Species in Iran; Wide Distribution of Aada1 and Arma, Infection# Genetics and Evolution (2018)

3. The Impact of Primer Sets on Detection of the Gene Encoding Biofilm-Associated Protein (Bap) in Acinetobacter Baumannii: In Silico and In Vitro Analysis, Letters in Applied Microbiology (2017)

4. Frequency of 16S Rrna Methylase and Aminoglycoside-Modifying Enzyme Genes Among Clinical Isolates of Acinetobacter Baumannii in Iran, Iranian Journal of Pathology (2017)

5. Screening of Mutator Phenotype in Clinical Strains of Acinetobacter Baumannii, Microbial Pathogenesis (2017)

7. The Diversity of Class B and Class D Carbapenemases in Clinical Acinetobacter Baumannii Isolates, Infezioni in Medicina (2018)

8. Molecular Characterization of Acinetobacter Baumannii Isolated From Ventilator-Associated Pneumonia and Burn Wound Colonization by Random Amplified Polymorphic Dna Polymerase Chain Reaction and the Relationship Between Antibiotic Susceptibility and Biofilm Production, Advanced Biomedical Research (2019)

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Style	Citing Format
MLA	Rad MR, et al.. "Antibacterial Activity of Gold Nanoparticles Conjugated by Aminoglycosides Against A. Baumannii Isolates From Burn Patients." Recent Patents on Anti-Infective Drug Discovery, vol. 13, no. 3, 2018, pp. 256-264.
APA	Rad MR, Kazemian H, Yazdani F, Monfared MRZ, Rahdar H, Javadi A, Kodori M (2018). Antibacterial Activity of Gold Nanoparticles Conjugated by Aminoglycosides Against A. Baumannii Isolates From Burn Patients. Recent Patents on Anti-Infective Drug Discovery, 13(3), 256-264.
Chicago	Rad MR, Kazemian H, Yazdani F, Monfared MRZ, Rahdar H, Javadi A, Kodori M. "Antibacterial Activity of Gold Nanoparticles Conjugated by Aminoglycosides Against A. Baumannii Isolates From Burn Patients." Recent Patents on Anti-Infective Drug Discovery 13, no. 3 (2018): 256-264.
Harvard	Rad MR et al. (2018) 'Antibacterial Activity of Gold Nanoparticles Conjugated by Aminoglycosides Against A. Baumannii Isolates From Burn Patients', Recent Patents on Anti-Infective Drug Discovery, 13(3), pp. 256-264.
Vancouver	Rad MR, Kazemian H, Yazdani F, Monfared MRZ, Rahdar H, Javadi A, et al.. Antibacterial Activity of Gold Nanoparticles Conjugated by Aminoglycosides Against A. Baumannii Isolates From Burn Patients. Recent Patents on Anti-Infective Drug Discovery. 2018;13(3):256-264.
BibTex	@article{ author = {Rad MR and Kazemian H and Yazdani F and Monfared MRZ and Rahdar H and Javadi A and Kodori M}, title = {Antibacterial Activity of Gold Nanoparticles Conjugated by Aminoglycosides Against A. Baumannii Isolates From Burn Patients}, journal = {Recent Patents on Anti-Infective Drug Discovery}, volume = {13}, number = {3}, pages = {256-264}, year = {2018} }
RIS	TY - JOUR AU - Rad MR AU - Kazemian H AU - Yazdani F AU - Monfared MRZ AU - Rahdar H AU - Javadi A AU - Kodori M TI - Antibacterial Activity of Gold Nanoparticles Conjugated by Aminoglycosides Against A. Baumannii Isolates From Burn Patients JO - Recent Patents on Anti-Infective Drug Discovery VL - 13 IS - 3 SP - 256 EP - 264 PY - 2018 ER -

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