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Mobile Genetic Elements Carrying Aminoglycoside Resistance Genes in Acinetobacter Baumannii Isolates Belonging to Global Clone 2 Publisher



Naderi G1 ; Talebi M2 ; Gheybizadeh R1 ; Seifi A3 ; Ghourchian S1 ; Rahbar M4 ; Abdollahi A5 ; Naseri A6 ; Eslami P7 ; Douraghi M1
Authors
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Authors Affiliations
  1. 1. Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Infectious Diseases, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Microbiology, Iranian Reference Health Laboratory Research Center, Ministry of Health and Medical Education, Tehran, Iran
  5. 5. Department of Pathology, Imam Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Laboratory Sciences, School of Paramedical Sciences, Iran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Microbiology, Milad Hospital, Tehran, Iran

Source: Frontiers in Microbiology Published:2023


Abstract

Aminoglycosides are used to treat infections caused by carbapenem-resistant Acinetobacter baumannii (CRAB) strains. However, resistance to aminoglycosides has increased remarkably in the last few years. Here, we aimed to determine the mobile genetic elements (MGEs) associated with resistance to aminoglycosides in the global clone 2 (GC2) A. baumannii. Among the 315 A. baumannii isolates, 97 isolates were identified as GC2, and 52 of GC2 isolates (53.6%) were resistant to all the aminoglycosides tested. The AbGRI3s carrying armA were detected in 88 GC2 isolates (90.7%), and of them, 17 isolates (19.3%) carried a new variant of AbGRI3 (AbGRI3ABI221). aphA6 was located in TnaphA6 of 30 isolates out of 55 aphA6-harboring isolates, and 20 isolates were found to harbor TnaphA6 on a RepAci6 plasmid. Tn6020 carrying aphA1b was detected in 51 isolates (52.5%), which was located within AbGRI2 resistance islands. The pRAY* carrying the aadB gene was detected in 43 isolates (44.3%), and no isolate was found to contain a class 1 integron harboring this gene. The GC2 A. baumannii isolates contained at least one MGE carrying the aminoglycoside resistance gene, located mostly either in the chromosome within AbGRIs or on the plasmids. Thus, it is likely that these MGEs play a role in the dissemination of aminoglycoside resistance genes in GC2 isolates from Iran. Copyright © 2023 Naderi, Talebi, Gheybizadeh, Seifi, Ghourchian, Rahbar, Abdollahi, Naseri, Eslami and Douraghi.
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