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Analysis of Virulence Genes and Accessory Gene Regulator (Agr) Types Among Methicillin-Resistant Staphylococcus Aureus Strains in Iran Publisher Pubmed



Cheraghi S1 ; Pourgholi L1 ; Shafaati M1 ; Fesharaki SH1 ; Jalali A2 ; Nosrati R3 ; Boroumand MA4
Authors
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Authors Affiliations
  1. 1. Department of Molecular Pathology, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Clinical Cardiovascular Research, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
  4. 4. Department of Pathology and Laboratory Medicine, Tehran Heart Center, Tehran University of Medical Sciences, North Karegar Ave., Tehran, 1411713138, Iran

Source: Journal of Global Antimicrobial Resistance Published:2017


Abstract

Objectives Methicillin-resistant Staphylococcus aureus (MRSA) strains are a major cause of hospital-acquired infections and are considered a serious public health concern. MRSA isolates have abundant virulence factors that are the basis for their pathogenicity. The accessory gene regulator (agr) locus co-ordinates the expression of these genes. The aim of this study was to determine the presence and frequency of various virulence genes encoding enterotoxins and adhesins as well as to identify agr specificity groups in MRSA isolates. Methods This descriptive study included a total of 296 MRSA strains isolated from clinical samples collected in Tehran Heart Center (Tehran, Iran) between October 2004 and March 2013. Following DNA extraction, PCR-based assays were used to evaluate the presence of various virulence genes. IBM SPSS Statistics for Windows v.21.0 was used for statistical analysis. Results The results indicated that the most frequent toxin genes were see (120/296; 40.5%), followed by sea (79/296; 26.7%); the other genes were encoded less frequently. The presence of seb and seh was not found in any of the isolates. Furthermore, the most frequent adhesin genes were clfA, spa, cna, map/eap and bbp, found in 281 (94.9%), 275 (92.9%), 267 (90.2%), 265 (89.5%) and 264 (89.2%) isolates, respectively. The majority of isolates belonged to agr group I (53.0%), followed by agr group III (1.4%). None of the isolates belonged to agr group II. Conclusions The relatively high frequency of various virulence genes suggests the emergence and pathogenic potential of MRSA isolates containing these genes in the study area. © 2017 International Society for Chemotherapy of Infection and Cancer
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