Ruthenium-Encapsulated Porphyrinic Organic Polymer As a Photoresponsive Oxidoreductase Mimetic Nanozyme for Colorimetric Sensing Publisher Pubmed



Talebi M¹ ; Dashtian K¹ ; Zaredorabei R¹ ; Amourizi F¹ ; Ghafuri H¹ ; Mahdavi M² Show Affiliations
Source: Langmuir Published:2023

Abstract

The advantages of porosity and stable unpaired electrons of porphyrinic organic polymers (POPs) with free radicals are exclusive and potentially practical functionalities and combining the semiconductor-like characteristics of these materials and metal ions has been an effective way to assemble an efficient photocatalytic system. Herein, a new ruthenium (Ru) ion-encapsulated porphyrinic organic polymer (POP/Ru) is facilely synthesized as a proper photoresponsive nanozyme with unique photo-oxidase properties. Surprisingly, the proposed POP/Ru revealed outstanding photoresponsive oxidase-mimicking activity due to the synergetic effect of the integration of Ru and π-electrons of POP, which boosts charge separation and transport. POP/Ru was applied to the oxidation of o-phenylenediamine (o-PDA) as a chromogenic probe for producing a colorimetric signal. The kinetic study reveals that these photo-oxidase mimics have a significant affinity for the o-PDA chromogenic agent owing to a lower Kmand superior Vmax. Further findings demonstrate that the presence of the l-arginine (l-Arg) target causes an inhibition effect on the photo-nanozymatic colorimetry of POP/Ru. This research develops the applications of the comprehensive colorimetric strategy for ultrasensitive l-Arg monitoring with a limit of detection (LOD) of 15.2 nM in the dynamic range of 4.0 nM-340 »μM and illuminates that the proposed photo-oxidase nanozyme as a visual strategy is feasible in l-Arg environmentally friendly colorimetric detection in juice samples. © 2023 American Chemical Society. All rights reserved.