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Photo-Responsive Oxidase-Like Nanozyme Based on a Vanadium-Docked Porphyrinic Covalent Organic Framework for Colorimetric L-Arginine Sensing Publisher Pubmed



Talebi M1 ; Dashtian K1 ; Zaredorabei R1 ; Ghafuri H2 ; Mahdavi M3 ; Amourizi F1
Authors
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Authors Affiliations
  1. 1. Research Laboratory of Spectrometry & Micro and Nano Extraction, Department of Chemistry, Iran University of Science and Technology, Tehran, 16846-13114, Iran
  2. 2. Biocatalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran, 16846-13114, Iran
  3. 3. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Analytica Chimica Acta Published:2023


Abstract

This study reports the development of a vanadium-docked porphyrinic covalent organic framework as a novel class of highly polar photoactive materials. Thanks to its extended π-electron conjugation and high chemical stabilities, this framework can serve as an oxidase-Like photo-nanozyme for photocatalytic oxidation of o-phenylenediamine (o-PDA) and a colorimetric substrate for the production of the yellow-colored oxidized o-PDA (o-PDAox). The physicochemical properties of the as-prepared photo-nanozyme were characterized by several analytical techniques. Its enhanced light harvesting and charge separation and transfer were also verified by electrochemical and spectroscopic analysis. This photo-nonenzymatic colorimetric assay was applied for the sensitive L-Arginine (L-Arg) detection as a typical amino acid in the linear range of 8.1 nM–330 μM with a limit of detection (LOD) of 3.5 nM. The findings of this research confirmed the safety and feasibility of the proposed photo-nonenzymatic colorimetric sensing strategy for the detection of L-Arg and other similar biomolecules in food samples. Kinetic investigation revealed that the photo-responsive oxidase mimic exhibits satisfactory Km (0.47 mM) and Vmax (42.0 μM/s) values. This work broadened our insight into the development of modified porphyrinic–COF–based visible light-responsive oxidase-like photo-nanozyme for environmentally friendly colorimetric biosensing. © 2023 Elsevier B.V.
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