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Tumor Suppression Effects of Myoepithelial Cells on Mice Breast Cancer Publisher Pubmed



Farhanji B1 ; Latifpour M2 ; Alizadeh AM2 ; Khodayari H2 ; Khodayari S2, 3 ; Khaniki M4 ; Ghasempour S2
Authors
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Authors Affiliations
  1. 1. Iranian Tissue Bank and Research Center, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Cancer Research Center, Tehran University of Medical Sciences, 1419733141, Tehran, Iran
  3. 3. Department of Pharmacology, Fasa University of Medical Sciences, Fasa, Iran
  4. 4. Pathology Department, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: European Journal of Pharmacology Published:2015


Abstract

Several studies have assumed that myoepithelial cells (MECs) loss may contribute to epithelial tumor induction and/or progression. We adopted an in vitro assay and a syngeneic mice breast cancer model with histological and molecular characteristics resembling human lesions to evaluate tumor suppression effects of MECs. Flow cytometric, cell viability, blood chemistry, transmission electron microscope, immunohistochemistry and qRT-PCR assays were performed at the end of the study. We demonstrated that MECs could significantly suppress the viability of cancer cells at different time points (P<0.05). At the end of the fourth and fifth weeks, treated mice had smaller tumor volume compared with control animals. Average tumor volume was significantly less in treated groups than control group at days 21 (0.38±0.19 vs. 1.99±0.13 cm 3 ), 28 (0.57±0.3 vs. 2.5±0.37 cm 3 ) and 35 (0.7±0.35 vs. 2.65±0.4 cm 3 ) after tumor cell injection (P<0.05). No hematological, hepatocellular, and renal toxicities were seen in MECs treated groups. Ultrastructural features revealed severe relationship between adjacent tumoral cells and loose interconnections of neoplastic cells in treated group. Immunohistochemical examinations of breast tumors showed high p63 and low alpha-smooth muscle actin protein expression in treated mice compared to control (P<0.05). MRNA expressions of TNF-α, smooth muscle-myosin heavy chain, connexin 43, and maspin were significantly up-regulated in breast tumor tissues in treated group compared to control (P<0.05). VEGF and alpha-smooth muscle actin mRNA expression were reduced in treated animals (P<0.05). The present study highlighted the potential tumor suppression effects of MECs on breast cancer in a typical animal model. © 2015 Elsevier B.V.
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