Therapeutic Effects of Dendrosomal Solanine on a Metastatic Breast Tumor Publisher Pubmed



Mohsenikia M¹ ; Farhangi B² ; Alizadeh AM² ; Khodayari H² ; Khodayari S³ ; Khori V² ; Arjmand Abbassi Y² ; Vesovic M⁴ ; Soleymani A¹ ; Najafi F⁵ Show Affiliations
Source: Life Sciences Published:2016

Abstract

Aims Our previous studies showed that alpha-solanine can inhibit tumor growth in cell culture and animal models of breast cancer. However, solanine is insoluble in common solvents; therefore, we developed a special nanoparticle with high-capacity solubility. The present study is aimed to deliberate the therapeutic effects of dendrosomal solanine (DNS) on a metastatic breast tumor in vitro and in vivo. Main methods After DNS preparation and dosing procedures, forty-five mice were equally divided into five groups to investigate the anti-metastatic effects of DNS on mammary tumor-bearing mice. Key findings Compared to solanine, DNS significantly suppressed the proliferation of 4 T1 cells in a dose- and time-dependent manner. DNS showed a remarkable safety rate of up to 10 mg/kg. A significant decrease in white blood-cell count was seen at 20 mg/kg DNS in comparison with control animals. Mice treated with DNS had smaller tumor volume (mm3) in comparison with control and solanine groups. Moreover, the incidence of the breast tumor metastases was about 67% in the control animals, where as solanine and DNS 1 mg/kg were about 22% and 0%, respectively. Furthermore, the number of metastases per mouse varied from one to three. The tissues of tumor, brain, liver, spleen, and lung showed higher expression levels of Bcl-2 but lower expression levels of Bax, MMP-2, MMP-9, mTOR, and Akt in DNS-treated mice than control and solanine groups. Significance The findings suggest that DNS has a more impactful therapeutic effect than solanine on 4 T1-induced breast tumorigenesis via influencing the tissue microenvironment. © 2016 Elsevier Inc. All rights reserved.