Natural Fruits, Flowers, Honey, and Honeybees Harbor Helicobacter Pylori-Positive Yeasts

Natural Fruits, Flowers, Honey, and Honeybees Harbor Helicobacter Pylori-Positive Yeasts Publisher Pubmed

Siavoshi F¹ ; Sahraee M¹ ; Ebrahimi H¹ ; Sarrafnejad A² ; Saniee P³

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1. Department of Microbiology, School of Biology, University College of Sciences, University of Tehran, Tehran, Iran
2. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3. Faculty of Life Science and Biotechnology, Department of Microbiology and Microbial Biotechnology, Shahid Beheshti University G. C, Tehran, Iran

Source: Helicobacter Published:2018

Abstract

Background: For controlling Helicobacter pylori infection in humans, its environmental reservoir should be determined. In this study, yeast isolates from an isolated village in Iran were studied for the intracellular occurrence of H. pylori. Materials and Methods: In this study, yeasts were isolated from 29 samples, including oral swabs from villagers (n = 7), flowers and fruits (n = 6), honey and honeybees (n = 12) and miscellaneous samples (4). Yeasts were classified into 12 RFLP groups and identified by amplification of 26S rDNA and sequencing. DNA extracted from the yeast cells was examined for the presence of H. pylori using PCR. Results: Of the 29 yeasts, 27 were members of different genera of Ascomycete. H. pylori was detected in 5 of 9 Candida (55.5%), 4 of 5 Komagataella (80%), 3 of 4 Pichia (100%), 2 of 2 Cytobasidia (100%), 2 of 2 Hansenia (100%), 1 of 1 Meyerozyma (100%) and 2 of 3 not sequenced (66.6%) yeasts. Distribution of 19 of 29 (65.5%) H. pylori-positive yeasts within 4 groups was as follows: 1 of 7(14.3%) in oral swabs, 5 of 6 (83.3%) in flowers and fruits, 10 of 12 (83.3%) in honey and the bee group and 3 of 4 (75%) in miscellaneous. Conclusions: Different genera of osmotolerant yeasts from flowers, fruits, honey, and honeybees contained H. pylori in their vacuole. High frequency of H. pylori-positive yeasts in these samples might be related to their high sugar content. Insects such as honeybees that facilitate transfer and easy access of these yeasts to nectars serve as the main reservoirs of these yeasts, playing an important role in their protection and dispersal. Accordingly, H. pylori inside these yeasts can be carried by honeybees to different sugar- and nutrient-rich environments. Sugar-rich environments and honeybees play an important role in distribution of H. pylori-positive yeasts in nature. © 2018 John Wiley & Sons Ltd

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Reza Malekzadeh (2)

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Style	Citing Format
MLA	Siavoshi F, et al.. "Natural Fruits, Flowers, Honey, and Honeybees Harbor Helicobacter Pylori-Positive Yeasts." Helicobacter, vol. 23, no. 2, 2018, pp. -.
APA	Siavoshi F, Sahraee M, Ebrahimi H, Sarrafnejad A, Saniee P (2018). Natural Fruits, Flowers, Honey, and Honeybees Harbor Helicobacter Pylori-Positive Yeasts. Helicobacter, 23(2), -.
Chicago	Siavoshi F, Sahraee M, Ebrahimi H, Sarrafnejad A, Saniee P. "Natural Fruits, Flowers, Honey, and Honeybees Harbor Helicobacter Pylori-Positive Yeasts." Helicobacter 23, no. 2 (2018): -.
Harvard	Siavoshi F et al. (2018) 'Natural Fruits, Flowers, Honey, and Honeybees Harbor Helicobacter Pylori-Positive Yeasts', Helicobacter, 23(2), pp. -.
Vancouver	Siavoshi F, Sahraee M, Ebrahimi H, Sarrafnejad A, Saniee P. Natural Fruits, Flowers, Honey, and Honeybees Harbor Helicobacter Pylori-Positive Yeasts. Helicobacter. 2018;23(2):-.
BibTex	@article{ author = {Siavoshi F and Sahraee M and Ebrahimi H and Sarrafnejad A and Saniee P}, title = {Natural Fruits, Flowers, Honey, and Honeybees Harbor Helicobacter Pylori-Positive Yeasts}, journal = {Helicobacter}, volume = {23}, number = {2}, pages = {-}, year = {2018} }
RIS	TY - JOUR AU - Siavoshi F AU - Sahraee M AU - Ebrahimi H AU - Sarrafnejad A AU - Saniee P TI - Natural Fruits, Flowers, Honey, and Honeybees Harbor Helicobacter Pylori-Positive Yeasts JO - Helicobacter VL - 23 IS - 2 SP - EP - PY - 2018 ER -

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