Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Apoptotic Effect of Resveratrol on Human T-All Cell Line Ccrf-Cem Is Unlikely Exerted Through Alteration of Bax and Bcl2 Promoter Methylation Publisher Pubmed



Zadi Heydarabad M1 ; Vatanmakanian M2 ; Abdolalizadeh J3 ; Mohammadi H1 ; Azimi A4 ; Mousavi Ardehaie R5 ; Movasaghpour A6 ; Farshdousti Hagh M3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  2. 2. Department of Hematology, Faculty of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  4. 4. Department of Basic Science, Maragheh University of Medical Sciences, Maragheh, Iran
  5. 5. Department of Biochemistry and Clinical Laboratory, Division of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  6. 6. Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Source: Journal of Cellular Biochemistry Published:2018


Abstract

One of the fundamental barriers leading to failure of leukemia therapy is the resistance against conventional chemotherapies, common modality used to cure leukemia. Having the potential to trigger apoptosis in various human leukemia cell lines, resveratrol is regarded as a robust agent in chemotherapy regimens. The current study was aimed to assess whether the apoptotic effect of resveratrol on T-cell acute lymphoblastic leukemia cell line, CCRF-CEM, is exerted through DNA methylation of BAX and BCL2 gene promoters. For this purpose, the CCRF-CEM cells were treated by resveratrol under standard cell culture. To analyze the promoter DNA methylation changes, we used methylation-specific polymerase chain reaction technique following the resveratrol treatment at different dosages and time intervals. Based on our previous study, the resveratrol treatment can trigger apoptosis in CCRF-CEM cell line via upregulation of apoptotic BAX gene and downregulation of antiapoptotic BCL2 gene. Despite these alterations in gene expression, the current study reveals no changes in DNA methylation patterns of subjected genes following the resveratrol treatment. Unchanged status of DNA methylation of BAX and BCL2 genes may suggest that resveratrol causes the gene expression changes through a distinct mechanism which requires further studies to be understood. © 2018 Wiley Periodicals, Inc.
Related Docs
1. Regulatory Effect of Resveratrol and Prednisolone on Mdr1 Gene Expression in Acute Lymphoblastic Leukemia Cell Line (Ccrf-Cem): An Epigenetic Perspective, Journal of Cellular Biochemistry (2018)
2. Micrornas Implications in the Onset, Diagnosis, and Prognosis of Osteosarcoma, Current Molecular Medicine (2021)
3. The Paradox of Th17 Cell Functions in Tumor Immunity, Cellular Immunology (2017)
4. Dynamic Changes of Epigenetic Signatures During Chondrogenic and Adipogenic Differentiation of Mesenchymal Stem Cells, Biomedicine and Pharmacotherapy (2017)
5. How Micrornas Affect the Pd-L1 and Its Synthetic Pathway in Cancer, International Immunopharmacology (2020)
6. Differential Role of Micrornas in Prognosis, Diagnosis, and Therapy of Ovarian Cancer, Biomedicine and Pharmacotherapy (2016)