Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Epidemiological Linkage of Vancomycin-Resistant Enterococcus Faecium From Different Sources in Ahwaz, Iran Publisher Pubmed



Arshadi M1 ; Shokoohizadeh L2 ; Douraghi M3 ; Owlia P4 ; Mashhadi R5 ; Soltani S3 ; Pourmand MR3, 5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, 6135715794, Iran
  2. 2. Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, 6517838678, Iran
  3. 3. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, 1417653761, Iran
  4. 4. Molecular Microbiology Research Center, Faculty of Medicine, Shahed University, Tehran, 331918651, Iran
  5. 5. Urology Research Center, Tehran University of Medical Sciences, Tehran, 1417653761, Iran

Source: FEMS Microbiology Letters Published:2019


Abstract

This study was set to determine the genetic linkage and the clonal relationship between vancomycin-resistant Enterococcus faecium (VREfm) isolates in three hospitals of Ahwaz city. In this study, 1050 samples were collected from various rectal swabs, hands of health care workers, environmental surfaces, medical equipment and 146 enterococci isolates from clinical sources of three hospitals from March to September 2015. Antimicrobial resistance patterns in VREfm were detected by disk diffusion method. Genetic linkages of VREfm strains were investigated by pulse field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) methods. Out of 366 enterococcal isolates, 163 Enterococcus faecium isolates were found to be resistant to vancomycin. PFGE and MLST analysis showed the presence of 79 pulsotypes and 11 sequence types (ST), respectively. In total, 90% of the isolates belonged to clonal complex 17 (CC17). Three new STs were reported for the first time in this study and ST80 was the predominant ST. We found a high prevalence of diverse VREfm with threatening antibiotic resistance patterns in all the studied sources with the dominance of CC17 VREfm strains in Ahwaz hospitals. Also, the results of typing method showed inter- and intra-hospital circulation of VREfm and similar pulsotypes and STs among different sources. © FEMS 2019. All rights reserved.
Related Docs
View other Related Docs
1. High Prevalence of Diverse Vancomycin Resistance Enterococcus Faecium Isolates in Clinical and Environmental Sources in Icu Wards in Southwest of Iran, Microbial Pathogenesis (2017)
2. Clinico-Epidemiological Profile of Vre Enterococcus Faecium in Shariati Hospital in Tehran, Online Journal of Health and Allied Sciences (2019)
3. Virulence Determinants and Antimicrobial Resistance Patterns of Vancomycin-Resistant Enterococcus Faecium Isolated From Different Sources in Southwest Iran, Iranian Journal of Public Health (2018)
4. Colonization Rate and Risk Factors of Vancomycin-Resistant Enterococci Among Patients Received Hematopoietic Stem Cell Transplantation in Shiraz, Southern Iran, International Journal of Organ Transplantation Medicine (2016)
5. Prevalence of Virulence Genes and Their Association With Biofilm Formation in Vre Faecium Isolates From Ahvaz, Iran, Journal of Infection in Developing Countries (2018)
6. Prevalence of Vancomycin-Resistant Enterococcus in Iran: A Systematic Review and Meta-Analysis, European Journal of Clinical Microbiology and Infectious Diseases (2016)
7. Characterization of Clostridioides Difficile Isolates Recovered From Hospitalized Patients and the Hospitals Environment and Air: A Multicenter Study, Anaerobe (2019)
8. Antibacterial Potential and Genetic Profile of Enterococcus Faecium Strains Isolated From Human Normal Flora, Microbial Pathogenesis (2016)
Experts (# of related papers)
View all Related Experts
Fereshteh Jabalameli (8)
Mohammad Emaneini (7)
Other Related Docs
9. High Frequency of Vancomycin Resistant Enterococcus Faecalis in Children: An Alarming Concern, Journal of Preventive Medicine and Hygiene (2016)
10. Prevalence of High-Level Gentamicin-Resistant Enterococcus Faecalis and Enterococcus Faecium in an Iranian Hospital, Journal of Preventive Medicine and Hygiene (2016)
11. Clonal Relatedness and Biofilm Formation of Oxa-23-Producing Carbapenem Resistant Acinetobacter Baumannii Isolates From Hospital Environment, Microbial Pathogenesis (2016)
12. Evaluation of Phenotypic and Genotypic Characteristics of Carbapnemases-Producing Enterobacteriaceae and Its Prevalence in a Referral Hospital in Tehran City, Iranian Journal of Pathology (2020)
13. Nasal Carriage Rate of Methicillin Resistant Staphylococcus Aureus Among Iranian Healthcare Workers: A Systematic Review and Meta-Analysis, Revista da Sociedade Brasileira de Medicina Tropical (2017)
14. Prevalence of Methicillin-Resistant Staphylococcus Aureus Isolated From Burn Patients in Iran: A Systematic Review and Meta-Analysis, Journal of Global Antimicrobial Resistance (2018)
15. Variable Spontaneous Mutation Rate in Clinical Strains of Multidrug-Resistant Acinetobacter Baumannii and Differentially Expressed Proteins in a Hypermutator Strain, Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis (2017)
16. Molecular Characterization of Staphylococcus Epidermidis Isolates Recovered From Patients Admitted to a Referral Hospital in Iran, Archives of Clinical Infectious Diseases (2017)
17. Molecular Study of Carbapenemase Genes in Clinical Isolates of Enterobacteriaceae Resistant to Carbapenems and Determining Their Clonal Relationship Using Pulsed-Field Gel Electrophoresis, Journal of Medical Microbiology (2017)
18. Virulence Factors, Antimicrobial Resistance Pattern and Molecular Analysis of Enterococcal Strains Isolated From Burn Patients, Microbial Pathogenesis (2016)
19. Molecular Epidemiology and Drug Resistance Pattern of Carbapenem-Resistant Klebsiella Pneumoniae Isolates From Iran, Microbial Drug Resistance (2019)
20. Antimicrobial Resistance Pattern, Virulence Determinants and Molecular Analysis of Enterococcus Faecium Isolated From Children Infections in Iran, BMC Microbiology (2019)
21. High Prevalence of Clostridiodes Diffiicle Pcr Ribotypes 001 and 126 in Iran, Scientific Reports (2020)
22. Detection of Fluoroquinolone Genes and Spread of Antibiotic Resistance Profile of Enterococcus Strains Isolated From Clinical Specimens, Gene Reports (2020)
23. Clonal Evolution Multi-Drug Resistant Acinetobacter Baumannii by Pulsed-Fi Eld Gel Electrophoresis, Indian Journal of Medical Microbiology (2015)
24. Molecular Cloning and Immunogenicity Evaluation of Ppic, Gele, and Vs87_01105 Proteins of Enterococcus Faecalis As Vaccine Candidates, Iranian Biomedical Journal (2019)
25. An Outbreak of Esbl-Producing Klebsiella Pneumoniae in an Iranian Referral Hospital: Epidemiology and Molecular Typing, Infectious Disorders - Drug Targets (2019)
26. Virulence and Antimicrobial Resistance of Enterococcus Faecium Isolated From Water Samples, Letters in Applied Microbiology (2015)
27. High Prevalence Rate of Microbial Contamination in Patient-Ready Gastrointestinal Endoscopes in Tehran, Iran: An Alarming Sign for the Occurrence of Severe Outbreaks, Microbiology Spectrum (2022)
28. Genotypic Diversity of Methicillin-Resistant Coagulase-Negative Staphylococci Isolated From Inpatients and Outpatients, Microbial Drug Resistance (2016)
29. Prevalence of Extended-Spectrum Β-Lactamase-Producing Klebsiella Pneumoniae: First Systematic Review and Meta-Analysis From Iran, Journal of Global Antimicrobial Resistance (2019)