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Lateral Flow Assays (Lfa) for Detection of Pathogenic Bacteria: A Small Point-Of-Care Platform for Diagnosis of Human Infectious Diseases Publisher Pubmed



Sohrabi H1 ; Majidi MR1 ; Fakhraei M1 ; Jahanbanesfahlan A2, 3 ; Hejazi M4 ; Oroojalian F5 ; Baradaran B6 ; Tohidast M6 ; Guardia MDL7 ; Mokhtarzadeh A6
Authors
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Authors Affiliations
  1. 1. Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, 51666 1647, Iran
  2. 2. Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  3. 3. Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  4. 4. Chronic Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Advanced Sciences and Technologies in Medicine, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
  6. 6. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  7. 7. Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, Burjassot, Valencia, 46100, Spain

Source: Talanta Published:2022


Abstract

Up-to-date diagnostics is globally improved by point-of-care testing (POCT) analysis and bedside research works. Development in POCT analysis has been provided mostly by forward-looking engineering technology for biosensing and sensing assessments. Lately, lateral flow assays (LFAs) have attracted a lot of interest as a result of their noteworthy benefits including cost-effectiveness, better portability, being operator friendly and rapid detection. This technique has been employed broadly for monitoring diverse biomarkers linked to ultrasensitive detection of pathogenic bacteria, ecological monitoring, consumer protection, and infectious diseases. LFA analyses established on qualitative and optical outcomes have boosted the objectivity and data efficiency of the assessments. Therefore, developing novel methods with the capability of providing reliable and quantitative information regarding a target analyte in a model and preserving the qualities of LFAs is of great necessity. In this review, the main principles of LFAs, challenges, and prospects for more development in this field in sensing pathogenic bacteria have been summarized. Subsequently, visually-read LFAs improvement to further progressive platforms have been explored by considering the prospects of this very flexible method for ultrasensitive detection of pathogenic bacteria. In addition, novel labeling methodologies, electrochemical and optical transducers are described. Also, recent developments in these detection methods elements in combination with other considered approaches have been highlighted. © 2022 Elsevier B.V.