Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Investigation of Specific Binding of Designed Oligodeoxynucleotide Decoys to Transcription Factors in Ht29 Cell Line Undergoing Epithelial–Mesenchymal Transition (Emt) Publisher Pubmed



Bigdelou Z1, 2 ; Johari B1, 2 ; Kadivar M3 ; Rismani E4 ; Asadi Z1, 2 ; Rahmati M1, 2 ; Saltanatpour Z5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Student Research Committee, Zanjan University of Medical Sciences, Zanjan, Iran
  2. 2. Department of Medical Biotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
  3. 3. Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran
  4. 4. Molecular Medicine Department, Pasteur Institute of Iran, Pasteur Avenue, Tehran, Iran
  5. 5. Genetics Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran

Source: Journal of Cellular Physiology Published:2019


Abstract

Expression of master transcriptional regulators of stem cells (Oct4 and Sox2) is associated with mediating tumor proliferation and tumor differentiation. The main goal of this study is the investigation of specific binding of designed Oct4–Sox2 transcription factors decoy oligodeoxynucleotides (ODNs) sequence to their nucleus-extracted proteins in HT29-ShE cells containing enriched cancer stem-like cells (SCLCs). First, gene expression of Oct4, Sox2, and E-cadherin revealed the overexpression of Oct4 and Sox2 and downregulation of E-cadherin in HT29-ShE cells compared with HT29 wild-type and HT29-ShC cells. Next, Oct4–Sox2 complex decoy ODNs were designed according to their elements in the promoter region of Sox2 gene. Then, the interactions of Oct4 and Sox2 proteins to designed ODNs were evaluated in silico. Finally, DNA–protein interactions of decoy ODNs and their corresponding proteins were examined by electrophoretic mobility shift assay (EMSA). Analysis of gel shift retardation assay admitted the specific binding of designed ODNs sequence to the nuclear extracted Oct4 and Sox2 proteins. The results will be a promising approach to target cancer stem cells for potential use in differentiation therapy before chemotherapy and radiotherapy of cancers. © 2019 Wiley Periodicals, Inc.
Related Docs
View other Related Docs
1. In Silico Studying of the Whole Protein Structure and Dynamics of Dickkopf Family Members Showed That N-Terminal Domain of Dickkopf 2 in Contrary to Other Dickkopfs Facilitates Its Interaction With Low Density Lipoprotein Receptor Related Protein 5/6, Journal of Biomolecular Structure and Dynamics (2019)
2. Role of Oct4–Sox2 Complex Decoy Oligodeoxynucleotides Strategy on Reverse Epithelial to Mesenchymal Transition (Emt) Induction in Ht29-She Encompassing Enriched Cancer Stem-Like Cells, Molecular Biology Reports (2020)
3. In Silico Mutagenesis in Recombinant Human Keratinocyte Growth Factor: Improvement of Stability and Activity in Addition to Decrement Immunogenicity, Journal of Molecular Graphics and Modelling (2020)
4. Hoxb7 and Hsa-Mir-222 As the Potential Therapeutic Candidates for Metastatic Colorectal Cancer, Recent Patents on Anti-Cancer Drug Discovery (2016)
5. Identification of New Dna Gyrase Inhibitors Based on Bioactive Compounds From Streptomyces: Structure-Based Virtual Screening and Molecular Dynamics Simulations Approaches, Journal of Biomolecular Structure and Dynamics (2020)
6. In Silico Enhancement of the Stability and Activity of Keratinocyte Growth Factor, Journal of Theoretical Biology (2017)
7. Enrichment of Cancer Stem-Like Cells by the Induction of Epithelial-Mesenchymal Transition Using Lentiviral Vector Carrying E-Cadherin Shrna in Ht29 Cell Line, Journal of Cellular Physiology (2019)
8. Role of Non-Coding Rnas in the Response of Glioblastoma to Temozolomide, Molecular Neurobiology (2025)
Experts (# of related papers)
View all Related Experts
Zohreh Saltanatpouri (5)
Arezoo Rasti (2)
Other Related Docs
9. Investigation of Biological Activities of Two Cultivars of Cicer Arietinum Proteins Mass Associated With Alzheimer's Disease, Proteins: Structure# Function and Bioinformatics (2023)
10. Decoding the Structure–Function Relationship of the Muramidase Domain in E. Coli O157.H7 Bacteriophage Endolysin: A Potential Building Block for Chimeric Enzybiotics, Protein Journal (2024)
11. Structure-Based Study of Immune Receptors As Eligible Binding Targets of Coronavirus Sars-Cov-2 Spike Protein, Journal of Molecular Graphics and Modelling (2021)
12. Increasing the Colon Cancer Cells Sensitivity Toward Radiation Therapy Via Application of Oct4–Sox2 Complex Decoy Oligodeoxynucleotides, Molecular Biology Reports (2020)
13. Optimization of Conformational Stability and Catalytic Efficiency in Chondroitinase Abc Ι by Protein Engineering Methods, Engineering in Life Sciences (2016)
14. Cytoplasmic Expression of Twist1, an Emt-Related Transcription Factor, Is Associated With Higher Grades Renal Cell Carcinomas and Worse Progression-Free Survival in Clear Cell Renal Cell Carcinoma, Clinical and Experimental Medicine (2018)
15. Preparation and Investigation of Indirubin-Loaded Sln Nanoparticles and Their Anti-Cancer Effects on Human Glioblastoma U87mg Cells, Cell Biology International (2019)
16. Mechanisms of Tumor Cell Resistance to the Current Targeted-Therapy Agents, Tumor Biology (2016)
17. Hbsag Mutations Related to Occult Hepatitis B Virus Infection in Hiv-Positive Patients Result in a Reduced Secretion and Conformational Changes of Hbsag, Journal of Medical Virology (2017)
18. Unraveling the Function of Epithelial-Mesenchymal Transition (Emt) in Colorectal Cancer: Metastasis, Therapy Response, and Revisiting Molecular Pathways, Biomedicine and Pharmacotherapy (2023)
19. Co-Expression of Cancer Stem Cell Markers Oct4 and Nanog Predicts Poor Prognosis in Renal Cell Carcinomas, Scientific Reports (2018)
20. Evaluation the Potential of Recombinant Anti-Cd3 Nanobody on Immunomodulatory Function, Molecular Immunology (2020)
21. Rational Design of Dkk3 Structure-Based Small Peptides As Antagonists of Wnt Signaling Pathway and in Silico Evaluation of Their Efficiency, PLoS ONE (2017)
22. Computational Design and Analysis of a Poly-Epitope Fusion Protein: A New Vaccine Candidate for Hepatitis and Poliovirus, International Journal of Peptide Research and Therapeutics (2020)
23. Anti-Proliferative and Anti-Metastatic Potential of High Molecular Weight Secretory Molecules From Probiotic Lactobacillus Reuteri Cell-Free Supernatant Against Human Colon Cancer Stem-Like Cells (Ht29-She), International Journal of Peptide Research and Therapeutics (2020)
24. Application of Nanotechnology in Targeting of Cancer Stem Cells: A Review, International Journal of Stem Cells (2019)
25. Exome Sequencing Reveals Novel Rare Variants in Iranian Familial Multiple Sclerosis: The Importance of Pold2 in the Disease Pathogenesis, Genomics (2021)
26. Radiosynthesis, Biological Evaluation, and Preclinical Study of a 68Ga-Labeled Cyclic Rgd Peptide As an Early Diagnostic Agent for Overexpressed Α V Β 3 Integrin Receptors in Non-Small-Cell Lung Cancer, Contrast Media and Molecular Imaging (2020)
27. Nano-Delivery System Targeting to Cancer Stem Cell Cluster of Differentiation Biomarkers, Journal of Controlled Release (2017)
28. The Penicillin Binding Protein 1A of Helicobacter Pylori, Its Amoxicillin Binding Site and Access Routes, Gut Pathogens (2021)
29. In Silico Design and Immunoinformatics Analysis of a Chimeric Vaccine Construct Based on Salmonella Pathogenesis Factors, Microbial Pathogenesis (2023)
30. Association of High Expression Levels of Sox2, Nanog, and Oct4 in Gastric Cancer Tumor Tissues With Progression and Poor Prognosis, Journal of Gastrointestinal Cancer (2020)
31. Competing Endogenous Rnas Regulatory Crosstalk Networks: The Messages From the Rna World to Signaling Pathways Directing Cancer Stem Cell Development, Heliyon (2024)
32. New Engineered Fusion Peptide With Dual Functionality: Antibacterial and Strong Binding to Hydroxyapatite, International Journal of Peptide Research and Therapeutics (2020)
33. Lung Cancer-Associated Brain Metastasis: Molecular Mechanisms and Therapeutic Options, Cellular Oncology (2017)
34. Role of the Bone Marrow Microenvironment in Drug Resistance of Hematological Malignances, Current Medicinal Chemistry (2022)
35. Isolation of a Novel Anti-Kdr3 Single-Chain Variable Fragment Antibody From a Phage Display Library, Iranian Journal of Allergy# Asthma and Immunology (2019)
36. Construction of a Recombinant B-Cell Epitope Vaccine Based on a Der P1-Derived Hypoallergen: A Bioinformatics Approach, Immunotherapy (2018)
37. In Silico Designing of a New Cysteine Analogue of Hirudin Variant 3 for Site Specific Pegylation, Research in Pharmaceutical Sciences (2017)
38. Evaluation of Novel 2-(Quinoline-2-Ylthio)Acetamide Derivatives Linked to Diphenyl-Imidazole As Α-Glucosidase Inhibitors: Insights From in Silico, in Vitro, and in Vivo Studies on Their Anti-Diabetic Properties, European Journal of Medicinal Chemistry (2024)
39. Epithelial-To-Mesenchymal Transition in Thyroid Cancer: A Comprehensive Review, Endocrine (2019)
40. The Evaluation of the N-Cadherin Promoter’S Ability to Block Emt by Specific Expression of Diphtheria Toxin in Emt-Induced A549 Cell Lines, Iranian Journal of Allergy# Asthma and Immunology (2024)
41. Computational Structure-Based Design of Antiviral Peptides As Potential Protein–Protein Interaction Inhibitors of Rabies Virus Phosphoprotein and Human Lc8, Heliyon (2025)
42. Unveiling the Structure of Gpi-Anchored Protein of Malassezia Globosa and Its Pathogenic Role in Pityriasis Versicolor, Journal of Molecular Modeling (2021)
43. Utilization of Whole Exome Sequencing in Lethal Form of Multiple Pterygium Syndrome: Identification of Mutations in Embryonal Subunit of Acetylcholine Receptor, International Journal of Molecular and Cellular Medicine (2019)
44. Structural Insights Into the Pathogenicity of Point Mutations in Human Acyl-Coa Dehydrogenase Homotetramers, Journal of Biological Physics (2024)
45. Impacts of the G145r Mutation on the Structure and Immunogenic Activity of the Hepatitis B Surface Antigen: A Computational Analysis, Hepatitis Monthly (2016)
46. Apoptosis Induction and Proliferation Inhibition by Silibinin Encapsulated in Nanoparticles in Mia Paca-2 Cancer Cells and Deregulation of Some Mirnas, Iranian Journal of Basic Medical Sciences (2020)
47. Harnessing Function of Emt in Cancer Drug Resistance: A Metastasis Regulator Determines Chemotherapy Response, Cancer and Metastasis Reviews (2024)
48. Pro-Tumorigenic Functions of Macrophages at the Primary, Invasive and Metastatic Tumor Site, Cancer Immunology# Immunotherapy (2020)
49. An Overview on the Complexity of Oct4: At the Level of Dna, Rna and Protein, Stem Cell Reviews and Reports (2021)
50. Tumor-Associated Macrophages and Epithelial–Mesenchymal Transition in Cancer: Nanotechnology Comes Into View, Journal of Cellular Physiology (2018)