Increasing the Colon Cancer Cells Sensitivity Toward Radiation Therapy Via Application of Oct4

Increasing the Colon Cancer Cells Sensitivity Toward Radiation Therapy Via Application of Oct4–Sox2 Complex Decoy Oligodeoxynucleotides Publisher Pubmed

Johari B^{1, 2, 3} ; Rezaeejam H^{4, 5} ; Moradi M⁶ ; Taghipour Z¹ ; Saltanatpour Z⁷ ; Mortazavi Y^{2, 9} ; Nasehi L^{1, 8}

Show Affiliations

1. Student Research Committee, Zanjan University of Medical Sciences, Zanjan, Iran
2. Department of Medical Biotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
3. Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
4. Department of Radiation Oncology, Vali-e-Asr Hospital, Zanjan University of Medical Sciences, Zanjan, Iran
5. Department of Radiology, School of Paramedical Sciences, Zanjan University of Medical Sciences, Zanjan, Iran
6. Department of Biotechnology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran
7. Stem Cell and Regenerative Medicine Center of Excellence, Tehran University of Medical Sciences, Tehran, Iran
8. Department of Medical Laboratory, School of Paramedical Sciences, Zanjan University of Medical Sciences, Zanjan, Iran
9. Cancer Gene Therapy Research Center, Zanjan University of Medical Sciences, Zanjan, Iran

Source: Molecular Biology Reports Published:2020

Abstract

Low sensitivity of cancer stem cells toward regular cancer therapy strategies is an important issue in the field of cancer remedy. The concept of cancer stem cell elimination has been a topic of interest in the field of molecular medicine for a long time. At the current study, it was aimed to elevate the sensitivity of cancer stem-like cells toward radiotherapy by treating with Oct4–Sox2 complex decoy oligodeoxynucleotides (ODNs). After treating HT29 and HT29-ShE cells with Oct4–Sox2 complex decoy ODNs, and analyzing the cellular uptake and localization of decoys, treated cells and control groups were subjected to irradiation by fractionated 6MV X-ray with a final dose of 2 Gy. Thereafter, the influence of radiotherapy on ODNs treated groups and control group was investigated on cell viability, cell cycle, apoptosis, colonosphere formation and scratch assay. Cellular uptake and localization assays demonstrated that decoy ODNs can efficiently be transfected to the cells and reside in subcellular compartment, where they pose their action on gene regulation. Post radiotherapy analysis indicated statistical significance in decoy ODNs treated cells by means of lower cell viability, cell cycle arrest in G2/M phase, increased cellular apoptosis, and reduced cell motility. Also, formed colonospheres were smaller in size and fewer in numbers. Considering the role of Oct4, and Sox2 transcription factors in signaling pathways of preserving stemness and inducing reverse EMT, application of decoy strategy could increase the sensitivity of cancer cells toward irradiation, which has a potential to eliminate the cancerous cells from tumors and support cancer treatment. © 2020, Springer Nature B.V.

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Style	Citing Format
MLA	Johari B, et al.. "Increasing the Colon Cancer Cells Sensitivity Toward Radiation Therapy Via Application of Oct4–Sox2 Complex Decoy Oligodeoxynucleotides." Molecular Biology Reports, vol. 47, no. 9, 2020, pp. 6793-6805.
APA	Johari B, Rezaeejam H, Moradi M, Taghipour Z, Saltanatpour Z, Mortazavi Y, Nasehi L (2020). Increasing the Colon Cancer Cells Sensitivity Toward Radiation Therapy Via Application of Oct4–Sox2 Complex Decoy Oligodeoxynucleotides. Molecular Biology Reports, 47(9), 6793-6805.
Chicago	Johari B, Rezaeejam H, Moradi M, Taghipour Z, Saltanatpour Z, Mortazavi Y, Nasehi L. "Increasing the Colon Cancer Cells Sensitivity Toward Radiation Therapy Via Application of Oct4–Sox2 Complex Decoy Oligodeoxynucleotides." Molecular Biology Reports 47, no. 9 (2020): 6793-6805.
Harvard	Johari B et al. (2020) 'Increasing the Colon Cancer Cells Sensitivity Toward Radiation Therapy Via Application of Oct4–Sox2 Complex Decoy Oligodeoxynucleotides', Molecular Biology Reports, 47(9), pp. 6793-6805.
Vancouver	Johari B, Rezaeejam H, Moradi M, Taghipour Z, Saltanatpour Z, Mortazavi Y, et al.. Increasing the Colon Cancer Cells Sensitivity Toward Radiation Therapy Via Application of Oct4–Sox2 Complex Decoy Oligodeoxynucleotides. Molecular Biology Reports. 2020;47(9):6793-6805.
BibTex	@article{ author = {Johari B and Rezaeejam H and Moradi M and Taghipour Z and Saltanatpour Z and Mortazavi Y and Nasehi L}, title = {Increasing the Colon Cancer Cells Sensitivity Toward Radiation Therapy Via Application of Oct4–Sox2 Complex Decoy Oligodeoxynucleotides}, journal = {Molecular Biology Reports}, volume = {47}, number = {9}, pages = {6793-6805}, year = {2020} }
RIS	TY - JOUR AU - Johari B AU - Rezaeejam H AU - Moradi M AU - Taghipour Z AU - Saltanatpour Z AU - Mortazavi Y AU - Nasehi L TI - Increasing the Colon Cancer Cells Sensitivity Toward Radiation Therapy Via Application of Oct4–Sox2 Complex Decoy Oligodeoxynucleotides JO - Molecular Biology Reports VL - 47 IS - 9 SP - 6793 EP - 6805 PY - 2020 ER -

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