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Frequencies of Six (Five Novel) Str Markers Linked to Tusc3 (Mrt7) or Nsun2 (Mrt5) Genes Used for Homozygosity Mapping of Recessive Intellectual Disability Publisher Pubmed



Ghadami S1 ; Mohammadi HM4 ; Malbin J5 ; Masoodifard M3 ; Sarhaddi AB3 ; Tavakkolybazzaz J2 ; Zeinali S1, 3
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Authors Affiliations
  1. 1. Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Pasteur St., Tehran, Iran
  2. 2. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Medical Genetics Lab., Kawsar Human Genetics Research Center, Tehran, Iran
  4. 4. Genetic Counseling Center, Shahid Fayazbakhsh Clinic, Tehran Welfare Organization, Tehran, Iran
  5. 5. Genetic Counseling Center, State Welfare Organization, Urmia, Iran

Source: Clinical Laboratory Published:2015


Abstract

Background: Non-syndromic autosomal recessive intellectual disability (NS-ARID) is an extremely heterogeneous genetic disorder. Therefore, to investigate these genes, more research is required. One approach to investigate the NS-ARID loci is homozygosity mapping which requires appropriate STR markers within or flanking the gene/s of interest. In this research, we aimed to find novel STRs for two common NS-ARID genes (TUSC3 and NSUN2) and, in addition, to identify allele frequencies of those STR markers. Methods: The study group included 119 unrelated healthy individuals. STR markers were investigated using the UCSC genome browser web site and SERV software. Genotyping was determined by multiplex PCR. Data were evaluated using Gene Mapper software. Allele frequencies and observed heterozygosity rates were calculated using PowerStatV12. Deviation from Hardy-Weinberg equilibrium and expected heterozygosity were assessed using the DNAView™ software. Results: In total, 56 alleles were detected. According to our research, D8TUSC3SU8.3 and D5NSUN2SU0.5 were the most informative STR markers in MRT7 and MRT5 loci, respectively and showed a high percentage of heterozygosity in Iranian population. The observed range of allele frequencies was from 3.4% to 32.4% and 0.8% to 18.9% for MRT5 and MRT7 loci, respectively. Further, we have evaluated other statistical surveys of these STR markers and discovered that all of the six listed STRs were informative and five meet the Hardy-Weinberg equilibrium for the tester group. Conclusions: Finding novel STRs, with high allele heterozygosity, is one of the most significant current finding in the present study for the two common NSARID genes. The recognized heterozygosity of these markers make MRT flanking STR markers very efficient to be used in diagnostic medical genetics labs or homozygosity mapping on NS-ARID.
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1. Multiplex Pcr Analysis of 17 (11 Novels) Str Markers Linked to Six Autosomal Recessive Intellectual Disability Genes in Iranian Population, Clinical Laboratory (2016)
2. Distinct Genetic Variation and Heterogeneity of the Iranian Population, PLoS Genetics (2019)
3. Mutations in the Histamine N-Methyltransferase Gene, Hnmt, Are Associated With Nonsyndromic Autosomal Recessive Intellectual Disability, Human Molecular Genetics (2015)
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