Erythrocytes Membrane Alterations Reflecting Liver Damage in Ccl 4 -Induced Cirrhotic Rats: The Ameliorative Effect of Naltrexone Pubmed



Kholari FS¹ ; Dehpour AR² ; Nourbakhsh M^{1, 3} ; Doustimotlagh AH¹ ; Bagherieh M¹ ; Golestani A¹ Show Affiliations
Source: Acta Medica Iranica Published:2016

Abstract

Cirrhosis is the consequence of chronic liver disease. Deleterious effects of oxidative stress on hepatocytes may be reflected in the erythrocyte membrane. Naltrexone (NTX) has been shown to attenuate hepatocellular injury in fibrotic animal models. The aim of this study was to investigate the progressive effect of CCl 4 on the liver and whether the improvement of liver cirrhosis can be monitored through alterations in the erythrocyte membrane. In this study, 84 male Wistar rats were divided into 4 groups and received reagents (i.p.) as follows: 1- CCl 4 , 2- NTX + CCl 4 , 3- Mineral Oil (M), and 4- NTX + M. After 2, 6 and 8 weeks, the blood and liver tissue samples were collected. Plasma enzyme activities, the content of erythrocyte GSH and some membrane compositions, including protein carbonyl, protein sulfhydryl, and malondialdehyde were assessed. After 6 and 8 weeks, plasma enzyme activities and the content of protein carbonyl were higher in CCl4 group significantly, as compared to other groups (P<0.001). NTX significantly diminished protein carbonyl and plasma enzyme activities (P<0.001). GSH did not change until the 6 th week. However, CCl4+NTX increased it significantly as compared to CCl 4 group (P<0.05). Protein sulfhydryl showed changes in NTX+CCl 4 group which indicated a significant increase in protein sulfhydryl content in a 6th week compared to CCl4 group (P<0.05). MDA did not show any significant alteration. CCl 4 -induced cirrhosis is accompanied by increased content of oxidative stress markers, especially protein carbonyl of RBC membrane and plasma enzyme activities. This study shows that the progression of liver cirrhosis and the ameliorative effect of NTX can be followed through alterations of these markers. © 2016 Tehran University of Medical Sciences. All rights reserved.