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The Efficacy of Lyticase and Β-Glucosidase Enzymes on Biofilm Degradation of Pseudomonas Aeruginosa Strains With Different Gene Profiles Publisher Pubmed



Banar M1 ; Emaneini M1 ; Beigverdi R1 ; Fanaei Pirlar R1 ; Node Farahani N1 ; Van Leeuwen WB2 ; Jabalameli F1
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Leiden Centre for Applied Bioscience, University of Applied Sciences Leiden, Leiden, 2333CR, Netherlands

Source: BMC Microbiology Published:2019


Abstract

Background: Pseudomonas aeruginosa is a nosocomial pathogen that causes severe infections in immunocompromised patients. Biofilm plays a significant role in the resistance of this bacterium and complicates the treatment of its infections. In this study, the effect of lyticase and β-glucosidase enzymes on the degradation of biofilms of P. aeruginosa strains isolated from cystic fibrosis and burn wound infections were assessed. Moreover, the decrease of ceftazidime minimum biofilm eliminating concentrations (MBEC) after enzymatic treatment was evaluated. Results: This study demonstrated the effectiveness of both enzymes in degrading the biofilms of P. aeruginosa. In contrast to the lyticase enzyme, β-glucosidase reduced the ceftazidime MBECs significantly (P < 0.05). Both enzymes had no cytotoxic effect on the A-549 human lung carcinoma epithelial cell lines and A-431 human epidermoid carcinoma cell lines. Conclusion: Considering the characteristics of the β-glucosidase enzyme, which includes the notable degradation of P. aeruginosa biofilms and a significant decrease in the ceftazidime MBECs and non-toxicity for eukaryotic cells, this enzyme can be a promising therapeutic candidate for degradation of biofilms in burn wound patients, but further studies are needed. © 2019 The Author(s).
3. Advanced Strategies for Combating Bacterial Biofilms, Journal of Cellular Physiology (2019)
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