Study of Virulence Genes and Related With Biofilm Formation in Pseudomonas Aeruginosa Isolated From Clinical Samples of Iranian Patients; a Systematic Review Publisher



Ghazalibina M¹ ; Morshedi K² ; Farahani RK³ ; Babadi M^{4, 5} ; Khaledi A^{4, 5} Show Affiliations
Source: Gene Reports Published:2019

Abstract

Biofilms are complex microbial communities containing microcolonies surrounded in a self-generated extracellular polysaccharide matrix. Biofilm cells show high resistance to environmental pressures such as antimicrobial agents than their planktonic form. This study aimed to evaluate virulence genes and related to biofilm formation in Pseudomonas aeruginosa isolated from clinical samples of Iranian patients by a systematic review. The keywords of biofilm formation, Pseudomonas aeruginosa, prevalence, virulence genes, and Iran with the help of Boolean operators (“AND” or “OR”) were used alone or individually or in combinations to conduct search process. The record search was done in the Web of Science, Cochrane Library, Scopus, PubMed, and Google Scholar databases. The search was limited to the articles published in English and Persian from 1 January 2005 to 31 March 2019. The cross-sectional studies reporting the prevalence of virulence genes related to biofilm formation in P. aeruginosa recovered from clinical samples of Iranian patients were included. STROBE checklist was used for assessment of study quality. In total, 14 studies were included in the present systematic review. The frequency of virulence genes varied from 38.6%–100%. The most virulence genes were reported exoA, ppyR, and algD with frequency 100%, followed by algU, exoY, and pslA with the prevalence of 98%, 95%, and 89.5%, respectively. Findings of the present systematic review showed that clinical isolates of P. aeruginosa had high virulence genes and the ability to form a biofilm. Also, most studies showed a significant correlation between biofilm formation and present virulence genes. © 2019 Elsevier Inc.