Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Differentiation of Wharton’S Jelly-Derived Mesenchymal Stem Cells Into Motor Neuron-Like Cells on Three-Dimensional Collagen-Grafted Nanofibers Publisher Pubmed



Bagher Z1 ; Azami M2 ; Ebrahimibarough S2, 3 ; Mirzadeh H4 ; Solouk A5 ; Soleimani M1, 6, 7 ; Ai J2, 3 ; Nourani MR8 ; Joghataei MT1, 6, 7
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Brain and Spinal Injury Research Center, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Polymer Engineering Faculty, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran
  5. 5. Biomedical Engineering Faculty, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran
  6. 6. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
  8. 8. Tissue Engineering Division, Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Source: Molecular Neurobiology Published:2016


Abstract

Cell transplantation strategies have provided potential therapeutic approaches for treatment of neurodegenerative diseases. Mesenchymal stem cells from Wharton’s jelly (WJMSCs) are abundant and available adult stem cells with low immunological incompatibility, which could be considered for cell replacement therapy in the future. However, MSC transplantation without any induction or support material causes poor control of cell viability and differentiation. In this study, we investigated the effect of the nanoscaffolds on WJMSCs differentiation into motor neuronal lineages in the presence of retinoic acid (RA) and sonic hedgehog (Shh). Surface properties of scaffolds have been shown to significantly influence cell behaviors such as adhesion, proliferation, and differentiation. Therefore, polycaprolactone (PCL) nanofibers were constructed via electrospinning, surface modified by plasma treatment, and grafted by collagen. Characterization of the scaffolds by means of ATR-FTIR, contact angel, and Bradford proved grafting of the collagen on the surface of the scaffolds. WJMSCs were seeded on nanofibrous and tissue culture plate (TCP) and viability of WJMSCs were measured by MTT assay and then induced to differentiate into motor neuron-like cells for 15 days. Differentiated cells were evaluated morphologically, and real-time PCR and immunocytochemistry methods were done to evaluate expression of motor neuron-like cell markers in mRNA and protein levels. Our results showed that obtained cells could express motor neuron biomarkers at both RNA and protein levels, but the survival and differentiation of WJMSCs into motor neuron-like cells on the PCL/collagen scaffold were higher than cultured cells in the TCP and PCL groups. Taken together, WJMSCs are an attractive stem cell source for inducing into motor neurons in vitro especially when grown on nanostructural scaffolds and PCL/collagen scaffolds can provide a suitable, three-dimensional situation for neuronal survival and differentiation that suggest their potential application towards nerve regeneration. © 2015, Springer Science+Business Media New York.
Related Docs
View other Related Docs
1. Cellular Activity of Wharton's Jelly-Derived Mesenchymal Stem Cells on Electrospun Fibrous and Solvent-Cast Film Scaffolds, Journal of Biomedical Materials Research - Part A (2016)
2. Induction of Human Umbilical Wharton’S Jelly-Derived Mesenchymal Stem Cells Toward Motor Neuron-Like Cells, In Vitro Cellular and Developmental Biology - Animal (2015)
3. Comparison of Capability of Human Bone Marrow Mesenchymal Stem Cells and Endometrial Stem Cells to Differentiate Into Motor Neurons on Electrospun Poly(Ε-Caprolactone) Scaffold, Molecular Neurobiology (2016)
4. Germline Cells Derived From Mesenchymal Stem Cells, With the Focus on Wharton's Jelly, Asian Pacific Journal of Reproduction (2018)
5. Evaluation of Motor Neuron-Like Cell Differentiation of Henscs on Biodegradable Plga Nanofiber Scaffolds, Molecular Neurobiology (2015)
6. Preparation of Fibrin Gel Scaffolds Containing Mwcnt/Pu Nanofibers for Neural Tissue Engineering, Journal of Biomedical Materials Research - Part A (2019)
7. Preparation of Bilayer Tissue-Engineered Polyurethane/Poly-L-Lactic Acid Nerve Conduits and Their in Vitro Characterization for Use in Peripheral Nerve Regeneration, Journal of Biological Engineering (2024)
8. A Simple, Rapid, and Efficient Method for Isolating Mesenchymal Stem Cells From the Entire Umbilical Cord, Cell Transplantation (2016)
Experts (# of related papers)
View all Related Experts
Somayeh Ebrahimi Barough (22)
Mahmoud Azami (7)
Other Related Docs
9. Electrospun Nerve Guide Scaffold of Poly(Ε-Caprolactone)/Collagen/Nanobioglass: An in Vitro Study in Peripheral Nerve Tissue Engineering, Journal of Biomedical Materials Research - Part A (2017)
10. Evaluation of Motor Neuron Differentiation Potential of Human Umbilical Cord Blood- Derived Mesenchymal Stem Cells, in Vitro, Journal of Chemical Neuroanatomy (2017)
11. Retinoic Acid As the Stimulating Factor for Differentiation of Wharton’S Jelly– Mesenchymal Stem Cells Into Hepatocyte-Like Cells, Avicenna Journal of Medical Biotechnology (2015)
12. In Vitro Differentiation of Human Ips Cells Into Neural Like Cells on a Biomimetic Polyurea, Molecular Neurobiology (2017)
13. Purmorphamine As a Shh Signaling Activator Small Molecule Promotes Motor Neuron Differentiation of Mesenchymal Stem Cells Cultured on Nanofibrous Pcl Scaffold, Molecular Neurobiology (2017)
14. Rapid Induction of Neural Differentiation in Human Umbilical Cord Matrix Mesenchymal Stem Cells by Camp-Elevating Agents, International Journal of Molecular and Cellular Medicine (2016)
15. Microrna-7 Promotes Neural Differentiation of Trabecular Meshwork Mesenchymal Stem Cell on Nanofibrous Scaffold, Journal of Cellular Biochemistry (2020)
16. Differentiation Potential of Human Chorion-Derived Mesenchymal Stem Cells Into Motor Neuron-Like Cells in Two- and Three-Dimensional Culture Systems, Molecular Neurobiology (2016)
17. Regulation of Stem Cell Fate by Nanomaterial Substrates, Nanomedicine (2015)
18. A Novel Polycaprolactone/Carbon Nanofiber Composite As a Conductive Neural Guidance Channel: An in Vitro and in Vivo Study, Progress in Biomaterials (2019)
19. Neural Stem/Progenitor Cell Transplantation for Spinal Cord Injury Treatment; a Systematic Review and Meta-Analysis, Neuroscience (2016)
20. Inhibitor of Pi3k/Akt Signaling Pathway Small Molecule Promotes Motor Neuron Differentiation of Human Endometrial Stem Cells Cultured on Electrospun Biocomposite Polycaprolactone/Collagen Scaffolds, Molecular Neurobiology (2017)
21. Bone Tissue Engineering Using Human Cells: A Comprehensive Review on Recent Trends, Current Prospects, and Recommendations, Applied Sciences (Switzerland) (2019)
22. Differentiation Potential of Human Bone Marrow Mesenchymal Stem Cells Into Motorneuron-Like Cells on Electrospun Gelatin Membrane, Journal of Molecular Neuroscience (2015)
23. Mesenchymal Stem Cells: Rising Concerns Over Their Application in Treatment of Type One Diabetes Mellitus, Journal of Diabetes Research (2015)
24. Transplantation of Mir-219 Overexpressed Human Endometrial Stem Cells Encapsulated in Fibrin Hydrogel in Spinal Cord Injury, Journal of Cellular Physiology (2019)
25. Tissue-Engineered Core-Shell Silk-Fibroin/Poly- L -Lactic Acid Nerve Guidance Conduit Containing Encapsulated Exosomes of Human Endometrial Stem Cells Promotes Peripheral Nerve Regeneration, ACS Biomaterials Science and Engineering (2023)
26. Enhanced Sciatic Nerve Regeneration by Human Endometrial Stem Cells in an Electrospun Poly (Ε-Caprolactone)/Collagen/Nbg Nerve Conduit in Rat, Artificial Cells# Nanomedicine and Biotechnology (2018)
27. Fabrication of Poly(L-Lactic Acid)/Chitosan Scaffolds by Solid-Liquid Phase Separation Method for Nerve Tissue Engineering: An in Vitro Study on Human Neuroblasts, Journal of Craniofacial Surgery (2019)
28. Stem-Cell Nanoengineering From Bench to Bed, Stem Cell Nanoengineering (2015)
29. Tissue-Engineered Regeneration of Hemisected Spinal Cord Using Human Endometrial Stem Cells, Poly Ε-Caprolactone Scaffolds, and Crocin As a Neuroprotective Agent, Molecular Neurobiology (2017)
30. Fabrication and Evaluation of Porous and Conductive Nanofibrous Scaffolds for Nerve Tissue Engineering, Journal of Materials Science: Materials in Medicine (2021)
31. Differentiation of Human Endometrial Stem Cells Into Endothelial-Like Cells on Gelatin/Chitosan/Bioglass Nanofibrous Scaffolds, Artificial Cells# Nanomedicine and Biotechnology (2016)
32. Mesenchymal Stem Cells in the Treatment of Amyotrophic Lateral Sclerosis, Current Stem Cell Research and Therapy (2016)
33. Oleic Acid Promotes the Expression of Neural Markers in Differentiated Human Endometrial Stem Cells, Journal of Chemical Neuroanatomy (2017)
34. Development of a Bioactive Scaffold Based on Ngf Containing Pcl/Chitosan Nanofibers for Nerve Regeneration, Biointerface Research in Applied Chemistry (2021)
35. Biomimetic Engineered Approaches for Neural Tissue Engineering: Spinal Cord Injury, Journal of Biomedical Materials Research - Part B Applied Biomaterials (2023)
36. Characterization of Wet-Electrospun Cellulose Acetate Based 3-Dimensional Scaffolds for Skin Tissue Engineering Applications: Influence of Cellulose Acetate Concentration, Cellulose (2016)
37. Biodegradable Functional Macromolecules As Promising Scaffolds for Cardiac Tissue Engineering, Polymers for Advanced Technologies (2022)
38. Tailoring Synthetic Polymeric Biomaterials Towards Nerve Tissue Engineering: A Review, Artificial Cells# Nanomedicine and Biotechnology (2019)
39. Cell Homing Strategy As a Promising Approach to the Vitality of Pulp-Dentin Complexes in Endodontic Therapy: Focus on Potential Biomaterials, Expert Opinion on Biological Therapy (2022)
40. Encapsulation of Curcumin Loaded Chitosan Nanoparticle Within Poly (Ε-Caprolactone) and Gelatin Fiber Mat for Wound Healing and Layered Dermal Reconstitution, International Journal of Biological Macromolecules (2020)
41. Stem Cells and Heart Tissue Regeneration, Nanomedicine for Ischemic Cardiomyopathy: Progress# Opportunities# and Challenges (2020)
42. Therapeutic Potential of Stem Cells for Treatment of Neurodegenerative Diseases, Biotechnology Letters (2020)
43. Development of Poly (Mannitol Sebacate)/Poly (Lactic Acid) Nanofibrous Scaffolds With Potential Applications in Tissue Engineering, Materials Science and Engineering C (2020)
44. Mechano-Transduction Signals Derived From Self-Assembling Peptide Nanofibers Containing Long Motif of Laminin Influence Neurogenesis in In-Vitro and In-Vivo, Molecular Neurobiology (2017)
45. Investigating the Neuroglial Differentiation Effect of Neuroblastoma Conditioned Medium in Human Endometrial Stem Cells Cultured on 3D Nanofibrous Scaffold, Journal of Biomedical Materials Research - Part A (2015)
46. Chimeric Self-Assembling Nanofiber Containing Bone Marrow Homing Peptide’S Motif Induces Motor Neuron Recovery in Animal Model of Chronic Spinal Cord Injury; an in Vitro and in Vivo Investigation, Molecular Neurobiology (2016)
47. Advanced Approaches to Regenerate Spinal Cord Injury: The Development of Cell and Tissue Engineering Therapy and Combinational Treatments, Biomedicine and Pharmacotherapy (2022)
48. Interplay Between Angiogenesis and Neurogenesis in Nerve Regeneration, Biomaterials for Vasculogenesis and Angiogenesis (2022)
49. Self-Assembling Peptide Nanofiber Containing Long Motif of Laminin Induces Neural Differentiation, Tubulin Polymerization, and Neurogenesis: In Vitro, Ex Vivo, and in Vivo Studies, Molecular Neurobiology (2016)
50. Development of Polyvinyl Alcohol Fibrous Biodegradable Scaffolds for Nerve Tissue Engineering Applications: In Vitro Study, International Journal of Polymeric Materials and Polymeric Biomaterials (2015)