Real-Time Pcr-Based Method for Detection of Aspergillus Spp in Bronchoalveolar Respiratory Samples Publisher



Sharifynia S¹ ; Tabarsi P¹ ; Bahrami N² ; Jamaati H³ ; Pourabdollah M³ ; Askari E³ ; Daustany M⁴ ; Emami M³ ; Zare S⁵ ; Hasani Y⁶ ; Mohamadnia A^{3, 7} Show Affiliations
Source: Minerva Pneumologica Published:2020

Abstract

Background: Some of the Aspergillus species are known to cause infectious diseases in humans; also, invasive aspergillosis is life-threatening for individuals with immunodeficiency. Thus, precise and rapid detection of Aspergillus and identification of its species are crucial. To detect Aspergillus and identify Aspergillus species in clinical samples using real-time polymerase chain reaction (PCR). Methods: in this study, a total of 100 Bal samples were collected from patients who referred to Masih Daneshvari Hospital and sent to laboratory to be cultured. PCR and real-time PCR were performed for samples using specific primers. Real-time PCR served as a gold standard. For final approval, some of the samples sent for sequencing. Results: Ninety-five of the samples reported positive for fungi in the culture, 16 of these were positive for Aspergillus. in Pcr, 94 samples were found positive for fungi, and 12 samples found to be Aspergillus. Finally, real-time Pcr reported 97 positive cases for fungi and 18 for Aspergillus. of these 18 cases, 6 Aspergillus flavus, 4 Aspergillus fumigatus and 3 Aspergillus terreus were isolated. Conclusions: Real-time PCR was shown to be more accurate and rapid technique in comparison with culture test and conventional PCR in identification of Aspergillus and its species with high accuracy. © 2020 Edizioni Minerva Medica