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Special Real-Time Pcr and Conventional Pcr for Detection of Rhino-Orbital-Cerebral Mucormycosis Publisher



Bahrami N1, 2 ; Jamaati H3 ; Farzanegan B4, 5 ; Nejad RA6 ; Mohammadi F2, 7 ; Naghdi M8 ; Kiaei SZF9 ; Mohamadnia A3, 10
Authors
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Authors Affiliations
  1. 1. Department of Tissue Engineering, Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University, Medical Sciences, Tehran, Iran
  2. 2. Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Department of Anaesthesiology, Critical Care Quality Improvement Research Center, Shahid Modarres Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  5. 5. Tracheal Diseases Research Center, National Research Institute, Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  6. 6. Department of Biology, Faculty of Sciences, Islamic Azad University, Tehran, Iran
  7. 7. Department of Oral and Maxillofacial Surgery, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  8. 8. Islamic Azad University of Medical Sciences, Tehran, Iran
  9. 9. Department of Pulmonary and Critical Care, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
  10. 10. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Journal of Cellular and Molecular Anesthesia Published:2024


Abstract

Background: Mucormycosis is an invasive fungal infection that occurs in immunodeficiency patients and belongs to infections caused by mucoral fungi, such as Rhizopus and Rhizomucor. Objectives: This study investigated the identification and detection of the mucor fungus in tissue samples and other samples by the real-time polymerase chain reaction (PCR) method. Methods: This was a cross-sectional-descriptive study. A total of 80 tissue samples were collected from referring patients to diagnose fungi with the opinion of a specialist doctor. After extracting deoxyribonucleic acid (DNA) from the samples, PCR and real-time PCR tests were performed using specific primers for mucor and universal-pan and compared to culture. The results were confirmed by sequencing. Results: In this study, 80 samples were examined. In the PCR method, 74 and 75 cases of fungi were confirmed from the DNA obtained from the colonies and tissues, respectively. By using a specific primer for mucor, 12 patients were reported as positive for mucor. Additionally, 76 and 77 cases of fungi were confirmed in the real-time PCR method from the DNA obtained from the colonies and tissues, respectively, and positive cases were reported. By using specific primers and the real-time PCR test for mucor, 16 cases of mucor were reported from colonies, and 15 cases of mucor were reported positive from tissues. Conclusions: This study showed that real-time PCR and PCR are high-speed and more accurate methods than the culture test in identifying its species and have acceptable results in clinical cases. © 2024 Shahid Beheshti University of Medical Sciences, Anesthesiology Research Center. All rights reserved.
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