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Effect of Genistein and L-Carnitine and Their Combination on Gene Expression of Hepatocyte Hmg-Coa Reductase and Ldl Receptor in Experimental Nephrotic Syndrome



Yousefinejad A1 ; Siassi F2 ; Mirshafiey A3 ; Eshraghian MR4 ; Koohdani F5 ; Javanbakht MH5 ; Sedaghat R6 ; Ramezani A7 ; Zarei M5 ; Djalali M5
Authors
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Authors Affiliations
  1. 1. Dept. of Nutrition, School of Public Health, Bushehr University of Medical Sciences, Bushehr, Iran
  2. 2. Dept. of Community Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Dept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Dept. of Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Dept. of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Dept. of Anatomy and Pathology, School of Medicine, Shahed University, Tehran, Iran
  7. 7. Dept. of Basic Sciences and Nutrition, School of Public Health, Mazandaran University of Medical Sciences, Sari, Iran

Source: Iranian Journal of Public Health Published:2015

Abstract

Background: Nephrotic syndrome is a disorder that leads to hyperlipidemia. L-carnitine and genistein can effect on lipid metabolism and the syndrome. In the present study, we have delved into the separate and the twin-effects of L-carnitine and genistein on the gene expressions of HMG-COA reductase and LDL receptor in experimental nephrotic syndrome. Methods: In this controlled experimental study, 50 male Sprague–Dawley rats were randomly divided into five groups: NC (normal-control), PC (patient-control), LC (L-carnitine), G (genistein), LCG (L-carnitine-genistein). Adri-amycin was used for inducing nephrotic syndrome and the spot urine samples and urine protein-to-creatinine ratio were measured. Hepatocytic RNA was extracted and real-time PCR was used for HMG-COA Reductase and LDL receptor gene Expression measurement. Results: The final weight of the patients groups were lower than the NC group (P=0.001), and weight gain of the NC group was higher than the other groups (P<0.001). The proteinuria and urine protein-to-creatinine ratio showed sig-nificant differences between PC group and LC, G and LCG groups at week 7 (P<0.001). The expression of HMG-COA Reductase mRNA down regulated in LC, G and LCG groups in comparison with PC group (P<0.001). ΔCT of LDLr mRNA showed significant differences between the PC group and the other patient groups (P<0.001). Conclusion: This study shows a significant decreasing (P<0.001) and non-significant increasing trend in HMG-COA Reductase and LDLr gene expression, respectively, and synergistic effect of L-carnitine and genistein on these genes in experimental nephrotic syndrome. © 2015, Iranian Journal of Public Health. All rights reserved.
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