Optimization of Cell/Tissue Culture of Linum Persicum for Production of Lignans Derivatives Including Podophyllotoxin

Optimization of Cell/Tissue Culture of Linum Persicum for Production of Lignans Derivatives Including Podophyllotoxin Publisher

Esfandiari M^{1, 2} ; Sharifi M¹ ; Mohamadyartoupkanlou F³ ; Hanaeeahwaz H⁴ ; Yousefzadi M⁵ ; Jafari A⁶ ; Hosseinzadeh S⁷ ; Soleimani M⁸

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1. Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, 14115-154, Iran
2. Department of Nanotechnology and Tissue Engineering, Stem Cell Technology Research Center, Tehran, Iran
3. Department of Chemical Engineering, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran
4. Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran
5. Department of Marine Biology, Hormozgan University, Bandar Abbas, Iran
6. Department of Phytochemistry, Faculty of Science, Yasouj University, Yasouj, Iran
7. Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
8. Department of Hematology, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran

Source: Plant Cell# Tissue and Organ Culture Published:2018

Abstract

Abstract: Linum persicum contains significant amount of lignans as podophyllotoxin that may show therapeutic effects against several types of metastatic cancers such as breast cancer. The aim of this study was to establish the cell culture of L. persicum for lignans production and evaluation for anti-carcinogenic activity on MCF7 cell line. Also, lignan contents of the cell, callus, and plantlets and also in different parts including; capsule, leave, stem and root of the wild plants were determined using HPLC technique. The highest podophyllotoxin in capsules and pinoresinol in leaf and root organs were observed. Shoot extract of the in-vitro plantlets have greater cytotoxic effect on breast cancer cells (IC50 = 5 µg/ml) and leads to significant raise in ratio of Bax/Bcl-2 value = 1.42 and Rbl1 gene expression that are well-known as apoptotic inducing agents. In conclusion, due to cytotoxic activity of in vitro plantlet extracts on MCF7 cell line with limited side-effects; the corresponding extracted component could be led to therapeutic approaches in future. Graphical abstract: [Figure not available: see fulltext.]. © 2018, Springer Science+Business Media B.V., part of Springer Nature.

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Style	Citing Format
MLA	Esfandiari M, et al.. "Optimization of Cell/Tissue Culture of Linum Persicum for Production of Lignans Derivatives Including Podophyllotoxin." Plant Cell# Tissue and Organ Culture, vol. 133, no. 1, 2018, pp. 51-61.
APA	Esfandiari M, Sharifi M, Mohamadyartoupkanlou F, Hanaeeahwaz H, Yousefzadi M, Jafari A, Hosseinzadeh S, Soleimani M (2018). Optimization of Cell/Tissue Culture of Linum Persicum for Production of Lignans Derivatives Including Podophyllotoxin. Plant Cell# Tissue and Organ Culture, 133(1), 51-61.
Chicago	Esfandiari M, Sharifi M, Mohamadyartoupkanlou F, Hanaeeahwaz H, Yousefzadi M, Jafari A, Hosseinzadeh S, Soleimani M. "Optimization of Cell/Tissue Culture of Linum Persicum for Production of Lignans Derivatives Including Podophyllotoxin." Plant Cell# Tissue and Organ Culture 133, no. 1 (2018): 51-61.
Harvard	Esfandiari M et al. (2018) 'Optimization of Cell/Tissue Culture of Linum Persicum for Production of Lignans Derivatives Including Podophyllotoxin', Plant Cell# Tissue and Organ Culture, 133(1), pp. 51-61.
Vancouver	Esfandiari M, Sharifi M, Mohamadyartoupkanlou F, Hanaeeahwaz H, Yousefzadi M, Jafari A, et al.. Optimization of Cell/Tissue Culture of Linum Persicum for Production of Lignans Derivatives Including Podophyllotoxin. Plant Cell# Tissue and Organ Culture. 2018;133(1):51-61.
BibTex	@article{ author = {Esfandiari M and Sharifi M and Mohamadyartoupkanlou F and Hanaeeahwaz H and Yousefzadi M and Jafari A and Hosseinzadeh S and Soleimani M}, title = {Optimization of Cell/Tissue Culture of Linum Persicum for Production of Lignans Derivatives Including Podophyllotoxin}, journal = {Plant Cell# Tissue and Organ Culture}, volume = {133}, number = {1}, pages = {51-61}, year = {2018} }
RIS	TY - JOUR AU - Esfandiari M AU - Sharifi M AU - Mohamadyartoupkanlou F AU - Hanaeeahwaz H AU - Yousefzadi M AU - Jafari A AU - Hosseinzadeh S AU - Soleimani M TI - Optimization of Cell/Tissue Culture of Linum Persicum for Production of Lignans Derivatives Including Podophyllotoxin JO - Plant Cell# Tissue and Organ Culture VL - 133 IS - 1 SP - 51 EP - 61 PY - 2018 ER -

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