Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Direct Bisulfite Sequencing and Methylation Specific Pcr to Detect Methylation of P15ink4b and F7 Genes in Coronary Artery Disease Patients



Yari M1 ; Movafagh A1 ; Sayad A1 ; Broumand MA2 ; Majidzadeha K3 ; Mirfakhraie R1 ; Khoshdel A4 ; Amini K4 ; Omrani MD1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Medical Genetics, Faculty of Medicine, University of Shahid Beheshti Medical Sciences, Tehran, Iran
  2. 2. Department of Molecular Pathology, Tehran Heart Center, University of Tehran Medical Sciences, Tehran, Iran
  3. 3. Department of Breast Cancer Genetics, Breast Cancer Research Center (BCRC), University of Tehran, Tehran, Iran
  4. 4. Department of Medical Sciences, Faculty of Medicine, University of AJA, Tehran, Iran

Source: Journal of Sciences# Islamic Republic of Iran Published:2016

Abstract

Genome-Wide Association Studies (GWAS) have identified genetic variants contributing to the risk of cardiovascular disease (CVD) at the chromosome 9p21 locus. The chromosome 9p21 is an important susceptibility locus for several multifactorial diseases like ischemic stroke, aortic aneurysm, type 2 diabetes mellitus and coronary artery disease (CAD). F7 gene because of its role in activating the extrinsic pathway by the exposure of tissue factor after plaque disruption is related to atherothrombosis. The aim of the present study was to evaluate methylation status of two CAD related genes, p15INK4b and F7, in Iranian patients with coronary artery disease (CAD). Thirty samples from 15 male and 15 female with diagnosed 3 vessels disease CAD and 60 Samples from 60 non-CAD controls who underwent coronary angiography was analyzed by MSP and direct sequencing. DNA methylation levels at p15INK4b gene increased significantly in CAD patients in comparison with control group (p-value<0.001). To quantitative analysis of methylation, direct bisulfite sequencing method revealed 6 methylated CpGs islands. No significant difference in F7 promoter methylation was observed between CAD patients and control group. Based on the findings of this study it is possible to assume, p15INK4b methylation is associated with pathophysiology of CAD.
Related Docs
1. Association Between Long Noncoding Rna Anril Expression Variants and Susceptibility to Coronary Artery Disease, International Journal of Molecular and Cellular Medicine (2018)
2. Association Between Rs10757274 and Rs2383206 Snps As Genetic Risk Factors in Iranian Patients With Coronary Artery Disease, Journal of Tehran University Heart Center (2017)
3. Genetic Variants Within Anril (Antisense Non Coding Rna in the Ink4 Locus) Are Associated With Risk of Psoriasis, International Immunopharmacology (2020)
4. New Epigenetic Players in Stroke Pathogenesis: From Non-Coding Rnas to Exosomal Non-Coding Rnas, Biomedicine and Pharmacotherapy (2021)
5. Next Generation Sequencing Applications for Cardiovascular Disease, Annals of Medicine (2018)
6. Association Between Abo Blood Group and Severity of Coronary Artery Disease in Unstable Angina, ARYA Atherosclerosis (2017)
Experts (# of related papers)
View all Related Experts
Mohammad Ali Boroumand (1)
Farnaz Khatami (1)