Isolation, Characterization, and Genome Analysis of a Broad Host Range Salmonella Phage Vb_Sens_Tums_E4: A Candidate Bacteriophage for Biocontrol

Isolation, Characterization, and Genome Analysis of a Broad Host Range Salmonella Phage Vb_Sens_Tums_E4: A Candidate Bacteriophage for Biocontrol Publisher Pubmed

Torkashvand N¹ ; Kamyab H¹ ; Shahverdi AR¹ ; Khoshayand MR² ; Sepehrizadeh Z¹

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1. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy & Biotechnology Research Center, Tehran University of Medical Sciences, PO Box 14155-6451, Tehran, Iran
2. Department of Food and Drug Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Source: Veterinary Research Communications Published:2023

Abstract

Salmonella enteritidis is one of the most important foodborne pathogens that cause numerous outbreaks worldwide. Some strains of Salmonella have become progressively resistant to antibiotics, so they could represent a critical threat to public health and have led to the use of alternative therapeutic approaches like phage therapy. In this study, a lytic phage, vB_SenS_TUMS_E4 (E4), was isolated from poultry effluent and characterized to evaluate its potential and efficacy for bio-controlling S. enteritidis in foods. Transmission electron microscopy revealed that E4 has a siphovirus morphotype, with an isometric head and non-contractile tail. Determining the host range showed that this phage can effectively infect different motile as well as non-motile Salmonella enterica serovars. The biological characteristics of E4 showed that it has a short latent period of about 15 min and a large burst size of 287 PFU/cell, and is also significantly stable in a broad range of pHs and temperatures. The E4 whole genome contains 43,018 bp and encodes 60 coding sequences (CDSs) but no tRNA genes. Bioinformatics analysis revealed that the genome of E4 lacks any genes related to lysogeny behavior, antibiotic resistance, toxins, or virulence factors. The efficacy of phage E4 as a bio-control agent was assessed in various foodstuffs inoculated with S. enteritidis at 4°C and 25°C, and the resulting data indicated that it could eradicate S. enteritidis after a very short time of 15 min. The findings of the present study showed that E4 is a hopeful candidate as a bio-control agent against S. enteritidis and has the potential to be used in various foodstuffs. © 2023, The Author(s), under exclusive licence to Springer Nature B.V.

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Ahmadreza Shahverdi (4)

Zargham Sepehrizadeh (4)

Style	Citing Format
MLA	Torkashvand N, et al.. "Isolation, Characterization, and Genome Analysis of a Broad Host Range Salmonella Phage Vb_Sens_Tums_E4: A Candidate Bacteriophage for Biocontrol." Veterinary Research Communications, vol. 47, no. 3, 2023, pp. 1493-1503.
APA	Torkashvand N, Kamyab H, Shahverdi AR, Khoshayand MR, Sepehrizadeh Z (2023). Isolation, Characterization, and Genome Analysis of a Broad Host Range Salmonella Phage Vb_Sens_Tums_E4: A Candidate Bacteriophage for Biocontrol. Veterinary Research Communications, 47(3), 1493-1503.
Chicago	Torkashvand N, Kamyab H, Shahverdi AR, Khoshayand MR, Sepehrizadeh Z. "Isolation, Characterization, and Genome Analysis of a Broad Host Range Salmonella Phage Vb_Sens_Tums_E4: A Candidate Bacteriophage for Biocontrol." Veterinary Research Communications 47, no. 3 (2023): 1493-1503.
Harvard	Torkashvand N et al. (2023) 'Isolation, Characterization, and Genome Analysis of a Broad Host Range Salmonella Phage Vb_Sens_Tums_E4: A Candidate Bacteriophage for Biocontrol', Veterinary Research Communications, 47(3), pp. 1493-1503.
Vancouver	Torkashvand N, Kamyab H, Shahverdi AR, Khoshayand MR, Sepehrizadeh Z. Isolation, Characterization, and Genome Analysis of a Broad Host Range Salmonella Phage Vb_Sens_Tums_E4: A Candidate Bacteriophage for Biocontrol. Veterinary Research Communications. 2023;47(3):1493-1503.
BibTex	@article{ author = {Torkashvand N and Kamyab H and Shahverdi AR and Khoshayand MR and Sepehrizadeh Z}, title = {Isolation, Characterization, and Genome Analysis of a Broad Host Range Salmonella Phage Vb_Sens_Tums_E4: A Candidate Bacteriophage for Biocontrol}, journal = {Veterinary Research Communications}, volume = {47}, number = {3}, pages = {1493-1503}, year = {2023} }
RIS	TY - JOUR AU - Torkashvand N AU - Kamyab H AU - Shahverdi AR AU - Khoshayand MR AU - Sepehrizadeh Z TI - Isolation, Characterization, and Genome Analysis of a Broad Host Range Salmonella Phage Vb_Sens_Tums_E4: A Candidate Bacteriophage for Biocontrol JO - Veterinary Research Communications VL - 47 IS - 3 SP - 1493 EP - 1503 PY - 2023 ER -

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