Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Oxaliplatin-Loaded Chitosan Nanoparticles Decorated With Cetuximab Single-Chain Variable Fragment for Human Colorectal Cancer Treatment Publisher



Falahzadeh K1 ; Esmaeili F2 ; Nematollahi L3 ; Bayat E3 ; Khoobi M4, 5 ; Mazloomi M1 ; Jalalvand M1 ; Majidi RF2 ; Negahdari B1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
  4. 4. Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Drug Design and Development Research Center, The Institute of Pharmaceutical Sciences, Tehran University of Medical Sciences, Tehran, Iran

Source: Cell Journal Published:2024


Abstract

Objective: Colorectal cancer (CRC) is the second leading cause of cancer-related deaths worldwide. Engineered biomolecules can be used as a targeted tool to deliver drugs directly to tumors that reduce the adverse effects of conventional treatments. We aimed to prepare non-targeted oxaliplatin-loaded chitosan nanoparticles (OXPT-CS NPs) and targeted OXPT-CS NPs decorated with cetuximab single-chain variable fragment (scFv) to send both NPs to epidermal growth factor receptor (EGFR) overexpressing HCT 116 cells, a human colorectal carcinoma cell line, for comparing their cytotoxicity. Materials and Methods: In this experimental study, OXPT-CS NPs were synthesized using a fluid system. Encapsulation efficiency percentage (EE%) and oxaliplatin release rate were evaluated. Western blot and cell-based ELISA confirmed scFv production and its binding ability to EGFR, respectively. The Fourier transform infrared spectroscopy (FTIR) determined the conjugation of scFv to OXPT-CS NPs. The NPs were characterized, and their toxicity against the HCT 116 cells was evaluated using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) and flow cytometry assays. Results: The EE% of OXPT-CS NPs was 93%, and the average diameters were 75.85 ± 8.81 nm and 92.48 ± 9.51 before and after scFv conjugation, respectively. The scFv was purified via affinity chromatography. The western blot method and cell-based ELISA revealed successful purification of scFv and its attachment to EGFR on HCT 116 cells. The FTIR analysis determined the interactions between the scFv and OXPT-CS NPs. According to MTT and flow cytometry results, the targeted delivery system significantly reduced HCT 116 cancer cell viability and increased apoptosis induction up to 99.8%. Conclusion: The scFv-OXPT-CS NPs demonstrated an increased cytotoxic function due to the presence of scFv in its formulation. This delivery system offers a promising method for delivering chemotherapy drugs to cancer cells. More research is needed on the best strategies for improving treatment efficacy by targeting cancer cells. © 2024 Royan Institute (ACECR). All rights reserved.
Related Docs
1. Cetuximab Scfv-Modified 5-Fu Loaded Chitosan Nanoparticles: “A Novel Therapeutic Platform.”, Current Pharmaceutical Biotechnology (2025)
2. Nanostructures for Site-Specific Delivery of Oxaliplatin Cancer Therapy: Versatile Nanoplatforms in Synergistic Cancer Therapy, Translational Oncology (2024)
3. Application of Polysaccharide Biopolymers As Natural Adsorbent in Sample Preparation, Critical Reviews in Food Science and Nutrition (2023)
4. Zeolites for Theranostic Applications, Journal of Materials Chemistry B (2020)
5. Prospects and Challenges of Anticancer Agents’ Delivery Via Chitosan-Based Drug Carriers to Combat Breast Cancer: A Review, Carbohydrate Polymers (2021)