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Upregulation of Fndc5 Gene Expression in C2c12 Cells After Single and Combined Treatments of Resveratrol and Atra Publisher Pubmed



Abeditaleb E1 ; Vahabi Z2, 3 ; Sekhavatimoghadam E4 ; Khedmat L5 ; Jazayeri S6 ; Sabooryaraghi AA1, 7
Authors
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Authors Affiliations
  1. 1. Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Geriatric Medicine, Ziaeian Hospital, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Memory and Behavioral Neurology Division, Roozbeh Hospital, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Cardiology, Ziaeian Hospital, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Health Management Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
  6. 6. Department of Nutrition and Biochemistry, School of Public Health, Iran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, 141613151, Iran

Source: Lipids in Health and Disease Published:2019


Abstract

Background: Irisin is a newly discovered myokine that secreted from skeletal muscle cells. Several studies showed that irisin involves in thermogenesis and increases the expression of browning markers such as uncoupling protein-1 that in turns induces the conversion of white adipose tissue to brown fat. Resveratrol (Res) and all-trans retinoic acid (ATRA) can also upregulate the expression of thermogenesis genes. In the present study, the effects of single and combined treatments of Res and ATRA on fibronectin type III domain containing 5 (FNDC5) gene expression was explored. Methods: The mouse myoblasts, C2C12 cells, were seeded in 6-well plastic plates and cultured in DMEM media. After differentiation, in a pilot study, C2C12 myotubes were treated with different concentrations of Res and ATRA for 12 h. The best result was obtained by treatment of 1and 25 μM of Res and 1 μM of ATRA. Then the main study was continued by single and combined treatment of these compounds at chosen concentration. After treatments, total RNA was extracted from C2C12 cells. Complementary DNA (cDNA) was generated by the cDNA synthesis kit and FNDC5 mRNA expression was evaluated by the real-time PCR method. Results: The FNDC5 gene expression in C2C12 myotubes of alone-treated with 1 μM, 25 μM Res and 10 μM ATRA did not change compared to vehicle group. However, in combination-treated the expression of FNDC5 gene was significantly increased compared to vehicle group. Conclusion: This is the first evidence that Res and ATRA can regulate FNDC5 gene expression in C2C12 myotubes. More investigations are necessary to explore the therapeutic effects of these nutrients in obesity, diabetes, cardiac and neurovascular disease. © 2019 The Author(s).
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