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Combined Effect of Retinoic Acid and Calcium on the in Vitro Differentiation of Human Adipose-Derived Stem Cells to Adipocytes Publisher Pubmed



Goudarzi F1 ; Sarveazad A2 ; Mahmoudi M3 ; Mohammadalipour A1, 7 ; Chahardoli R3 ; Malekshah OM4 ; Karimi Gorgani S5 ; Sabooryaraghi AA6
Authors
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Authors Affiliations
  1. 1. Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
  2. 2. Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Cellular Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ, United States
  5. 5. Health Information Management Department, Paramedical School, Kermanshah University of Medical Sciences, Kermanshah, Iran
  6. 6. Department of Immunology, School of Public Health, Tehran University of Medical sciences, Tehran, Iran
  7. 7. Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Archives of Physiology and Biochemistry Published:2018


Abstract

Context: It has been shown that adipogenesis can be modulated by factors such as all-trans retinoic acid (ATRA) and calcium. Objective: To determine, the combined effect of ATRA and calcium on the differentiation of human adipose-derived stem cells (hADSCs). Methods: Mesenchymal stem cells (MSCs) were differentiated into the adipocytes by 0.5 and 1 µM of ATRA and 5 and 10 mM calcium separately or in combination. After MTS assay the differentiation of MSCs to adipocyte was evaluated, Oil Red O staining, GLUT4 concentration and gene expression of PPARG2, adiponectin, and GLUT4 were measured by Real-Time PCR. Results: Except 10 mM calcium treated group, other groups and more significantly combination treatments could reduce all adipocyte markers compared to the control. Conclusion: These results suggest that ATRA and calcium together have significant inhibitory effect on adipogenesis that can be helpful for finding new mechanisms to prevent or control the adipogenesis. © 2017 Informa UK Limited, trading as Taylor & Francis Group.
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