Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Effects of Oleuropein on Pentylenetetrazol-Induced Seizures in Mice: Involvement of Opioidergic and Nitrergic Systems Publisher Pubmed



Rahimi N1, 2 ; Delfan B3 ; Motamedgorji N1, 2 ; Dehpour AR1, 2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, PO Box 13145-784, Tehran, Iran
  3. 3. Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

Source: Journal of Natural Medicines Published:2017


Abstract

Oleuropein, a well-known olive polyphenol, has been shown to mediate neuroprotection in Alzheimer’s disease and cerebral ischemia. We investigated the effects of oleuropein on pentylenetetrazole (PTZ)-induced seizures in male NMRI mice, with diazepam as the standard drug. We also examined the possible involvement of opioidergic/nitrergic pathways in the probable effects of oleuropein. Intraperitoneal (i.p.) administration of different doses of oleuropein (10, 20 and 30 mg/kg) significantly increased the seizure threshold 60 min prior to induction of seizure, in a dose-dependent manner. Administration of naltrexone (10 mg/kg, i.p.), an opioid receptor antagonist, completely reversed the anticonvulsant effects of oleuropein (10 mg/kg). On the other hand, the anticonvulsant effect of oleuropein (10 mg/kg) was blocked by a non-effective dose of nonspecific inhibitor of nitric oxide synthase (NOS), L-NAME (1 and 10 mg/kg, i.p) and a selective inhibitor of neuronal NOS, 7-nitroindazole (30 mg/kg, i.p.). However, the nitric oxide precursor, l-arginine (30 and 60 mg/kg, i.p.) potentiated the anticonvulsant activity of oleuropein (10 mg/kg). A selective inducible NOS inhibitor, aminoguanidine (100 mg/kg, i.p.) did not change the anticonvulsant activity of oleuropein. It seems that the opioidergic system and constitutive neuronal NOS may be involved in the anticonvulsant properties of oleuropein. © 2017, The Japanese Society of Pharmacognosy and Springer Japan.
Other Related Docs
9. Anticonvulsant Effect of Melatonin Through Atp-Sensitive Channels in Mice, Fundamental and Clinical Pharmacology (2020)