Evaluation of Interleukin-12 Receptor Β1 and Interferon Gamma Receptor 1 Deficiency in Patients With Disseminated Bcg Infection

Evaluation of Interleukin-12 Receptor Β1 and Interferon Gamma Receptor 1 Deficiency in Patients With Disseminated Bcg Infection Publisher Pubmed

Pourakbari B¹ ; Hosseinpour Sadeghi R¹ ; Mahmoudi S¹ ; Parvaneh N¹ ; Keshavarz Valian S² ; Mamishi S^{1, 3}

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1. Pediatric Infectious Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran
2. School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Infectious Diseases, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Allergologia et Immunopathologia Published:2019

Abstract

Introduction: Disseminated BCG infections among other complications of Bacillus Calmette–Guerin (BCG) vaccine are rare and have occurred in children with immunodeficiency disorders such as mendelian susceptibility to mycobacterial disease (MSMD) which could be due to defects in some elements of IL-12/IFN-γ axis. MSMD-causing mutations have been identified in 10 genes during the last two decades. Among them, mutations in the IL12Rβ1 and IFNγR1 genes constitute about 80% of recorded cases of MSMD syndrome. The aim of this study was to investigate IL-12Rβ1 and IFN-γR1 deficiencies in patients with disseminated BCG infection. Methods: This study was performed on 31 children with disseminated BCG infections who referred to children's medical center. Whole blood cell culture was performed in presence of BCG, IL-12 and IFN-γ stimulators. The supernatants were assayed for IFN-γ and IL-12p70 by ELISA method. In order to evaluate IL12Rβ1 and IFN-γR1 receptors expression, flow cytometry staining was performed on the patients’ T-cells stimulated with PHA. Results: Flow cytometry staining of 31 Iranian patients with disseminated BCG infections with the average age of 43 months showed lack of the expression of IL-12Rβ1 and IFN-γR1 genes in PHA-T-cells of the nine and one patients, respectively in whom the incomplete production of IFN-γ and IL-12 was reported by ELISA. Among these 10 patients, eight cases had related parents (80%). Conclusion: It is recommended that to avoid BCG complications, screening be performed for MSMD before BCG inoculation in individuals with positive family history of primary immunodeficiency diseases and inhabitants of areas with high frequency of consanguinity. © 2018 SEICAP

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Style	Citing Format
MLA	Pourakbari B, et al.. "Evaluation of Interleukin-12 Receptor Β1 and Interferon Gamma Receptor 1 Deficiency in Patients With Disseminated Bcg Infection." Allergologia et Immunopathologia, vol. 47, no. 1, 2019, pp. 38-42.
APA	Pourakbari B, Hosseinpour Sadeghi R, Mahmoudi S, Parvaneh N, Keshavarz Valian S, Mamishi S (2019). Evaluation of Interleukin-12 Receptor Β1 and Interferon Gamma Receptor 1 Deficiency in Patients With Disseminated Bcg Infection. Allergologia et Immunopathologia, 47(1), 38-42.
Chicago	Pourakbari B, Hosseinpour Sadeghi R, Mahmoudi S, Parvaneh N, Keshavarz Valian S, Mamishi S. "Evaluation of Interleukin-12 Receptor Β1 and Interferon Gamma Receptor 1 Deficiency in Patients With Disseminated Bcg Infection." Allergologia et Immunopathologia 47, no. 1 (2019): 38-42.
Harvard	Pourakbari B et al. (2019) 'Evaluation of Interleukin-12 Receptor Β1 and Interferon Gamma Receptor 1 Deficiency in Patients With Disseminated Bcg Infection', Allergologia et Immunopathologia, 47(1), pp. 38-42.
Vancouver	Pourakbari B, Hosseinpour Sadeghi R, Mahmoudi S, Parvaneh N, Keshavarz Valian S, Mamishi S. Evaluation of Interleukin-12 Receptor Β1 and Interferon Gamma Receptor 1 Deficiency in Patients With Disseminated Bcg Infection. Allergologia et Immunopathologia. 2019;47(1):38-42.
BibTex	@article{ author = {Pourakbari B and Hosseinpour Sadeghi R and Mahmoudi S and Parvaneh N and Keshavarz Valian S and Mamishi S}, title = {Evaluation of Interleukin-12 Receptor Β1 and Interferon Gamma Receptor 1 Deficiency in Patients With Disseminated Bcg Infection}, journal = {Allergologia et Immunopathologia}, volume = {47}, number = {1}, pages = {38-42}, year = {2019} }
RIS	TY - JOUR AU - Pourakbari B AU - Hosseinpour Sadeghi R AU - Mahmoudi S AU - Parvaneh N AU - Keshavarz Valian S AU - Mamishi S TI - Evaluation of Interleukin-12 Receptor Β1 and Interferon Gamma Receptor 1 Deficiency in Patients With Disseminated Bcg Infection JO - Allergologia et Immunopathologia VL - 47 IS - 1 SP - 38 EP - 42 PY - 2019 ER -

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