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A Trivalent Vaccine Consisting of “Flagellin A+B and Pilin” Protects Against Pseudomonas Aeruginosa Infection in a Murine Burn Model Publisher Pubmed



Hashemi FB1 ; Behrouz B1, 2 ; Irajian G3 ; Laghaei P3 ; Korpi F3 ; Fatemi MJ2
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Burn Research Center, Hazrat Fatima Hospital, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

Source: Microbial Pathogenesis Published:2020


Abstract

Pseudomonas aeruginosa is a common nosocomial pathogen in burn patients, and rapidly achieves antibiotic resistance, and thus, developing an effective vaccine is critically important for combating P. aeruginosa infection. Flagella and pili play important roles in colonization of P. aeruginosa at the burn wound site and its subsequent dissemination to deeper tissue and organs. In the present study, we evaluated protective efficacy of a trivalent vaccine containing flagellins A and B (FlaA + FlaB) + pilin (PilA) in a murine burn model of infection. “FlaA + FlaB + PilA” induced greater protection in P. aeruginosa murine burn model than the single components alone, and it showed broad immune protection against P. aeruginosa strains. Immunization with “FlaA + FlaB + PilA” induced strong opsonophagocytic antibodies and resulted in reduced bacterial loads, systemic IL-12/IL-10 cytokine expression, and increased survival after challenge with three times lethal dose fifty (LD50) of P. eruginosa strains. Moreover, the protective efficacy of “FlaA + FlaB + PilA” vaccination was largely attributed to specific antibodies. Taken together, these data further confirm that the protective effects of “FlaA + FlaB + PilA” vaccine significantly enhance efficacy compared with antibodies against either mono or divalent antigen, and that the former broadens the coverage against P. eruginosa strains that express two of the three antigens. © 2019
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