Antibodies Raised Against Divalent Type B Flagellin and Pilin Provide Effective Immunotherapy Against Pseudomonas Aeruginosa Infection of Mice With Burn Wounds

Antibodies Raised Against Divalent Type B Flagellin and Pilin Provide Effective Immunotherapy Against Pseudomonas Aeruginosa Infection of Mice With Burn Wounds Publisher Pubmed

Saffari M¹ ; Behbood S¹ ; Irajian G² ; Khorshidi A¹ ; Moniri R¹ ; Behrouz B³

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1. Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran
2. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
3. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Biologicals Published:2017

Abstract

Burn wound infections caused by multidrug-resistant Pseudomonas aeruginosa strains are a serious challenge to therapy because of the complex pathogenesis and paucity of new effective antibiotics. Therefore, there is renewed interest in developing antibody-based therapeutic strategies. Immunotherapy strategies typically target selected virulence factors that are expressed by the majority of clinical strains of P. aeruginosa, particularly because virulence factors mediate infection. Here we used a murine model of burn wound infection to evaluate the efficacy of antibodies raised against the divalent type b flagellin and PilA (flagellin b + PilA), as acute virulence factors, to prevent and treat infection. Antibodies to flagellin b + PilA exhibited superior synergistic effects that improved opsono-phagocytosis and cell invasion compared with antibodies to each monovalent flagellin b or PilA. Further, when used for prophylaxis, the antibodies against flagellin b + PilA and combined therapeutic and prophylactic regimens markedly improved the survival of mice infected with disparate P. aeruginosa strains from 91.6% to 100% compared with treatment using imipenem. Therefore, antibodies against flagellin b + PilA interfere with the activities of their respective cognate individual target antigens and enhance coverage against clinical strains of P. aeruginosa that may not express one of these two virulence factors. © 2016 International Alliance for Biological Standardization

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Style	Citing Format
MLA	Saffari M, et al.. "Antibodies Raised Against Divalent Type B Flagellin and Pilin Provide Effective Immunotherapy Against Pseudomonas Aeruginosa Infection of Mice With Burn Wounds." Biologicals, vol. 45, no. , 2017, pp. 20-26.
APA	Saffari M, Behbood S, Irajian G, Khorshidi A, Moniri R, Behrouz B (2017). Antibodies Raised Against Divalent Type B Flagellin and Pilin Provide Effective Immunotherapy Against Pseudomonas Aeruginosa Infection of Mice With Burn Wounds. Biologicals, 45(), 20-26.
Chicago	Saffari M, Behbood S, Irajian G, Khorshidi A, Moniri R, Behrouz B. "Antibodies Raised Against Divalent Type B Flagellin and Pilin Provide Effective Immunotherapy Against Pseudomonas Aeruginosa Infection of Mice With Burn Wounds." Biologicals 45, no. (2017): 20-26.
Harvard	Saffari M et al. (2017) 'Antibodies Raised Against Divalent Type B Flagellin and Pilin Provide Effective Immunotherapy Against Pseudomonas Aeruginosa Infection of Mice With Burn Wounds', Biologicals, 45(), pp. 20-26.
Vancouver	Saffari M, Behbood S, Irajian G, Khorshidi A, Moniri R, Behrouz B. Antibodies Raised Against Divalent Type B Flagellin and Pilin Provide Effective Immunotherapy Against Pseudomonas Aeruginosa Infection of Mice With Burn Wounds. Biologicals. 2017;45():20-26.
BibTex	@article{ author = {Saffari M and Behbood S and Irajian G and Khorshidi A and Moniri R and Behrouz B}, title = {Antibodies Raised Against Divalent Type B Flagellin and Pilin Provide Effective Immunotherapy Against Pseudomonas Aeruginosa Infection of Mice With Burn Wounds}, journal = {Biologicals}, volume = {45}, number = {}, pages = {20-26}, year = {2017} }
RIS	TY - JOUR AU - Saffari M AU - Behbood S AU - Irajian G AU - Khorshidi A AU - Moniri R AU - Behrouz B TI - Antibodies Raised Against Divalent Type B Flagellin and Pilin Provide Effective Immunotherapy Against Pseudomonas Aeruginosa Infection of Mice With Burn Wounds JO - Biologicals VL - 45 IS - SP - 20 EP - 26 PY - 2017 ER -

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