Effect of Microrna-21 Transfection on In-Vitro Differentiation of Human Naive Cd4+ T Cells to Regulatory T Cells Pubmed



Namdari H^{1, 2} ; Ghayedi M¹ ; Hadjati J¹ ; Rezaei F³ ; Kalantar K² ; Rahimzadeh P⁴ ; Salehi E¹ Show Affiliations
Source: Iranian Journal of Allergy# Asthma and Immunology Published:2017

Abstract

Regulatory T cells (Tregs) are important components of the immune system that modulate responses of other cells... These cells are involved in peripheral tolerance mechanisms, so defect in development and function of these cells can result in autoimmune disease. Increasing evidence supports the role of microRNAs-21 (miR-21) in the regulation of forkhead box P3 ( Foxp3 ) expression in Tregs. We aimed to determine whether miR-21 transfection to naive CD4+ T cells can be useful in generation of iTregs in-vitro. We investigated in-vitro differentiation of miR-21-transfected naive CD4+ T cells to iTregs and compared these iTregs to cytokine-differentiated iTregs and control group. We showed that expression of Foxp3, Transforming growth factor beta (TGF-aand Interleukin-10 (IL-10) are increased in iTregs generated after miR-21 transfection in comparison with cytokine-differentiated iTregs and control group. Our findings demonstrate that miR-21 has positive role in in-vitro generation of induced regulatory T-cells (iTregs). © Summer 2017, Iran J Allergy Asthma Immunol.