Arthrocen, an Avocado-Soy Unsaponifiable Agent, Improves Acetic Acid-Induced Colitis in Rat by Inhibition of Nf-Kb Signaling Pathway Publisher Pubmed



Goudarzi R¹ ; Partoazar A^{2, 3} ; Mumtaz F^{2, 3} ; Yousefimanesh H³ ; Abdollahi A⁴ ; Dehpour A^{2, 3} ; Rashidian A^{2, 3} Show Affiliations
Source: Journal of Food Biochemistry Published:2020

Abstract

The goal of the current study was to evaluate the anti-inflammatory effect of Arthrocen against acetic acid-induced colitis in rats. Acute inflammation was produced through intrarectal administration of 2 ml diluted acetic acid (4%) solution. All interventions were carried out for 5 days after colitis induction. Arthrocen was administered orally at doses of 30, 60, and 120 mg kg−1 day−1. Then, macroscopic and microscopic studies were performed. Myeloperoxidase (MPO) activity and tumor necrosis factor-α (TNF-α) activity were measured by biochemical and ELISA methods, respectively. Immunohistochemistry was done to investigate the expression of pNF-κB. The results of this study demonstrated that Arthrocen reduced macroscopic and microscopic damage compared to the acetic acid group. Furthermore, Arthrocen decreased the activity of MPO and TNF-α as well as the protein expression of pNF-kB in rat colon tissue. The results of the current study revealed the anti-inflammatory activity of Arthrocen in acetic acid mediated colon inflammation through suppressing the NF-κB pathway. Practical applications: Inflammatory bowel disease (IBD) is an immune-mediated chronic relapsing disorder affecting the gastrointestinal tract (GIT) characterized by chronic bowel inflammation. A plant-based dietary supplement containing avocado and soy unsaponifiable extracts in a ratio of 1:2 is known as Arthrocen. Arthrocen can be used as a complementary drug beside current drugs in clinical trials for the treatment of IBD. © 2020 Wiley Periodicals LLC.