Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Effect of Brain-Derived Neurotrophic Factor on Sperm Function, Oxidative Stress and Membrane Integrity in Human Publisher Pubmed



Najafi A1 ; Amidi F1 ; Sedighi Gilani MA2 ; Moawad AR3, 4 ; Asadi E1 ; Khanlarkhni N1 ; Fallah P5 ; Rezaiian Z5 ; Sobhani A1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Urology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Departments of Obstetrics and Gynecology, Surgery, McGill University, Montreal, QC, Canada
  4. 4. Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
  5. 5. Infertility Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran

Source: Andrologia Published:2017


Abstract

Oxidative stress has negative impacts on the clinical outcomes of assisted reproduction techniques. The brain-derived neurotrophic factor (BDNF) promotes the viability of nerve cells and is known to decrease oxidative stress and apoptosis in different cells. The aim of this study was to evaluate the effect of BDNF treatment on human sperm functions that are known to be essential for fertilisation. Our findings showed that treatment of human spermatozoa with 0.133 nM BDNF significantly increased the percentages of both total (P = 0.001) and progressive (P < 0.01) motile sperm cells compared to those observed in the nontreated (control) group. We also showed that the mean fluorescence intensity of DCFH-DA, as an indicator of intracellular reactive oxygen species, was significantly lower (P < 0.05) in spermatozoa treated with BDNF compared to the control group. Treatment of spermatozoa with BDNF significantly decreased the percentages of both dead (P = 0.001) and apoptotic-like sperm cells (P < 0.05) compared to the control group. On the other hand, BDNF treatment significantly increased the percentage of viable sperm cells compared to the control (P = 0.001). In conclusion, BDNF has protective effects against oxidative stress in spermatozoa and could improve sperm functions that are essential for sperm–egg fusion and subsequent fertilisation. © 2016 Blackwell Verlag GmbH
Related Docs
View other Related Docs
1. Supplementation of Freezing and Thawing Media With Brain-Derived Neurotrophic Factor Protects Human Sperm From Freeze-Thaw-Induced Damage, Fertility and Sterility (2016)
2. Melatonin Affects Membrane Integrity, Intracellular Reactive Oxygen Species, Caspase3 Activity and Akt Phosphorylation in Frozen Thawed Human Sperm, Cell and Tissue Research (2018)
3. Evaluation of Ros-Tac Score and Dna Damage in Fertile Normozoospermic and Infertile Asthenozoospermic Males, Urology Journal (2017)
4. Protective Features of Resveratrol on Human Spermatozoa Cryopreservation May Be Mediated Through 5’ Amp-Activated Protein Kinase Activation, Andrology (2017)
5. Comparing Reactive Oxygen Species and Dna Fragmentation in Semen Samples of Unexplained Infertile and Healthy Fertile Men, Irish Journal of Medical Science (2018)
6. Effect of Sulforaphane on Apoptosis, Reactive Oxygen Species and Lipids Peroxidation of Human Sperm During Cryopreservation, Cryobiology (2021)
7. Effect of Brain-Derived Neurotrophic Factor (Bdnf) on Sperm Quality of Normozoospermic Men, Human Fertility (2018)
8. Evaluation of the Nox5 Protein Expression and Oxidative Stress in Sperm From Asthenozoospermic Men Compared to Normozoospermic Men, Journal of Endocrinological Investigation (2019)
Experts (# of related papers)
View all Related Experts
Fardin Amidi (9)
Maryam Shabani Nashtaee (5)
Other Related Docs
9. The Effect of Astaxanthin on Motility, Viability, Reactive Oxygen Species, Apoptosis, and Lipid Peroxidation of Human Spermatozoa During the Freezing-Thawing Process, Biopreservation and Biobanking (2022)
10. Curcumin Nanomicelle Improves Semen Parameters, Oxidative Stress, Inflammatory Biomarkers, and Reproductive Hormones in Infertile Men: A Randomized Clinical Trial, Phytotherapy Research (2018)
11. Effect of Trolox on Sperm Quality in Normozospermia and Oligozospermia During Cryopreservation, Cryobiology (2016)
12. Bdnf and Its Signaling in Cancer, Journal of Cancer Research and Clinical Oncology (2023)
13. The Potential Relationship Between Different Human Female Reproductive Disorders and Sperm Quality in Female Genital Tract, Reproductive Sciences (2022)
14. Effect of Alpinia Officinarum Hance Rhizome Extract on Spermatogram Factors in Men With Idiopathic Infertility: A Prospective Double-Blinded Randomised Clinical Trial, Andrologia (2019)
15. Leptin and Prolactin Reduce Cryodamage in Normozoospermic Human Semen Samples During Cryopreservation; [La Leptina Y La Prolactina Reducen El Criodano En Muestras De Semen Humano Normozoospermico Durante La Criopreservacion], Revista Internacional de Andrologia (2023)
16. The Effect of Aminoguanidine on Sperm Motility and Mitochondrial Membrane Potential in Varicocelized Rats, Iranian Journal of Basic Medical Sciences (2016)
17. Sex Steroids-Induced Neurogenesis in Adult Brain: A Better Look at Mechanisms and Mediators, Hormone Molecular Biology and Clinical Investigation (2021)
18. Comparison of the Efficiency of Magnetic-Activated Cell Sorting (Macs) and Physiological Intracytoplasmic Sperm Injection (Picsi) for Sperm Selection in Cases With Unexplained Infertility, Journal of Reproduction and Infertility (2022)
19. Saffron Supplementation Ameliorates Oxidative Damage to Sperm Dna Following a 16-Week Low-To-Intensive Cycling Training in Male Road Cyclists, Journal of Functional Foods (2016)
20. Supplementation of Sperm Freezing Medium With Myoinositol Improve Human Sperm Parameters and Protects It Against Dna Fragmentation and Apoptosis, Cell and Tissue Banking (2019)
21. Male Factors: The Role of Sperm in Preimplantation Embryo Quality, Reproductive Sciences (2021)
22. The Challenge of Human Spermatozoa Proteome: A Systematic Review, Journal of Reproduction and Infertility (2017)
23. Controversy and Consensus on Indications for Sperm Dna Fragmentation Testing in Male Infertility: A Global Survey, Current Guidelines, and Expert Recommendations, World Journal of Men's Health (2023)
24. Protective Effects of Hesperetin on the Quality of Sperm, Apoptosis, Lipid Peroxidation, and Oxidative Stress During the Process of Cryopreservation: An Experimental Study, International Journal of Reproductive BioMedicine (2021)
25. The Association Between Dietary Fat and Mineral Intake With Semen Parameters: A Cross-Sectional Study in Infertile Men, International Journal of Reproductive BioMedicine (2022)
26. Evaluation of Intracellular Anion Superoxide Level, Heat Shock Protein A2 and Protamine Positive Spermatozoa Percentages in Teratoasthenozoospermia, International Journal of Reproductive BioMedicine (2017)
27. The Effect of Noise Stress on Adult Male Rat Sperm Parameters and the Protective Effect of Hydroalcoholic Cinnamomum Verum Extract: An Experimental Study, Iranian Red Crescent Medical Journal (2018)
28. The Effect of Omega-3 Fatty Acids, Epa, And/Or Dha on Male Infertility: A Systematic Review and Meta-Analysis, Journal of Dietary Supplements (2019)
29. Ovarian Tissue Culture in the Presence of Vegf and Fetuin Stimulates Follicle Growth and Steroidogenesis, Journal of Endocrinology (2017)
30. The Effects of Maternal Separation Stress Experienced by Parents on Male Reproductive Potential in the Next Generation, Heliyon (2020)
31. Oxidative Stress, Inflammatory Reactions and Apoptosis Mediated the Negative Effect of Chronic Stress Induced by Maternal Separation on the Reproductive System in Male Mice, Reproductive Biology (2019)