Prevalence of Human Papilloma Virus (Hpv) Genotypes Between Outpatients Males and Females Referred to Seven Laboratories in Tehran, Iran Publisher



Rezaee Azhar I^{1, 2} ; Yaghoobi M² ; Mossalaeie MM³ ; Kollaee Darabi A⁴ ; Nejadeh AH⁵ ; Jamshidi M⁵ ; Ahani A⁶ ; Karkhane Mahmoodi M⁷ ; Ghalichi L⁸ ; Shabanzadeh A^{1, 2} ; Ataeipirkooh A⁹ ; Marjani A¹ ; Khamseh A^{1, 10} ; Shafiei M^{1, 2} Show All Authors Show Affiliations
Source: Infectious Agents and Cancer Published:2022

Abstract

Background: Human papilloma virus (HPV) causes the most common sexually-transmitted infection especially among sexually-active individuals. The aim of study was to characterize the molecular characterization of HPV genotypes between 5176 female and male patients. Methods: HPV DNA was extracted from genital swabs of the study participants and amplified by Real Time Polymerase Chain Reaction (PCR). Genotyping was performed for 2525 cases using REALQUALITY RQ-Multi HPV Detection Kit for the identification of 14 high risk (HR) and 2 low risk (LR) HPV genotypes. Demographic figures were analyzed in correlation with virological data statistically. Results: Out of 5176 cases from 7 laboratories, 2727 (53%) were positive for HPV, of which. 2372(87%) women and 355 (13%) men were HPV positive. However, in an intra-gender analysis, positive rate was higher in men (355/637, 55.7%) than in women (2372/4539, 52%; P value 0.007). HPV positive patients were younger than negative individuals. Positive rate was higher among age categories 20–40. Genotyping was performed for 2525 cases. Out of 1219 (48%) patients who contained single genotypes, 566 (22%) and 653 (26%) harboured HR and LR genotypes, respectively. In females and males, 1189 (54%) and 117 (37%) contained multiple genotypes. No substantial associations were found between different age categories and HR/LR and multiple genotypes distribution. Conclusion: The prevalence of HPV infection in both genders was high. However, men had a higher rate of infection. These observations highlighted the necessity for a plan for targeted education to younger population in the society as well as application of infection control measures against HPV infection, especially in terms of general population mass HPV vaccination. © 2022, The Author(s).