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Rapid Hemostasis by Nanofibers of Polyhydroxyethyl Methacrylate/Polyglycerol Sebacic Acid: An in Vitro / in Vivo Study Publisher



Varshosaz J1 ; Choopannejad Z1 ; Minaiyan M2 ; Kharazi AZ3
Authors
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Authors Affiliations
  1. 1. Novel Drug Delivery Systems Research Center, Department of Pharmaceutics, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Biomaterials, Tissue Engineering and Nanotechnology, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Applied Polymer Science Published:2021


Abstract

Porous nanofibers were prepared from a combination of polyglycerol sebacate (PGS) and polyhydroxyethyl methacrylate (PHEMA) and loaded with tranexamic acid (TA) using the electrospinning method. The nanofibers were optimized for their morphology, diameter size, porosity, TA loading, release profile and mechanical behavior. Their cytotoxicity was studied based on 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide assay on L929 cells. The hemostasis control on a tail-cut model in rats was investigated. The best formulation contained 35% of the total polymers, 20% PGS and 10% TA in proportion to the total polymer quantity. These nanofibers had 64% porosity, 8.59% water sorption and 1.47% weight loss after 28 days with no cytotoxicity on the L929 cells. TA loaded nanofibers showed significantly less bleeding volume compared to the other groups, but no significant difference in bleeding time was seen with the blank nanofibers. In other words, the blank nanofibers alone had a hemostatic effect. TA loaded nanofibers were effective in bleeding control and hemorrhagic situations by reducing bleeding time and volume. © 2020 Wiley Periodicals LLC