Phytochemical Screening of Alstonia Venenata Leaf and Bark Extracts and Their Antimicrobial Activities Publisher Pubmed



Bagheri G¹ ; Ayatollahi SA^{2, 3} ; Ramirezalarcon K⁴ ; Salehi B⁵ ; Mohammadi R² ; Rahmani A² ; Martorell M^{4, 6} ; Sharifirad J² ; Sheibani M⁷ ; Amarowicz R⁸
Source: Cellular and Molecular Biology Published:2020

Abstract

Alstonia venenata is a plant commonly found in South India and used in traditional medicine. The aim of this study was to characterize the phytochemicals present in A. venenata leaf and bark extracts and study their antimicrobial activities. Solvent extractions with Soxhlet apparatus of leaves and bark were obtained using hexane, benzene, isopropanol, methanol, and water. The crude extracts were concentrated and screened for qualitative phytochemical content and analyzed by thin layer chromatography. The antibacterial, antifungal and antiviral activities of crude extracts were measured by in vitro methods. Alkaloids, carbohydrates, tannins, phenolic compounds, terpenoids, cardiac glycosides and amino acids were found in the different crude extracts analyzed. Isopropanol extracts showed antifungal activity and it was more pronounced in the bark extract than the leaf extract. Moreover, the isopropanol extract exhibited antibacterial and antiviral activity. In conclusion, the leaves and bark of A. venenata have antimicrobial components which are more present in the isopropanol fraction. Copyright: © 2020 by the C.M.B. Association. All rights reserved.