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Identification of Isoflavonoids in Antioxidant Effective Fraction of Arum Rupicola Boiss. Leaves Publisher



Tofighi Z1, 2 ; Shahpar Y2 ; Taheri A3 ; Tavakoli S4 ; Asatouri R5 ; Eftekhari M6 ; Vazirian M2 ; Ardekani MRS2 ; Lamardi SNS7
Authors
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Authors Affiliations
  1. 1. Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Medicinal Chemistry, Pharmaceutical Branch Islamic Azad University, Tehran, Iran
  4. 4. Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
  5. 5. Department of Biology, Science and Research Branch Islamic Azad University, Tehran, Iran
  6. 6. Department of Pharmacognosy and Pharmaceutical Biology, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran
  7. 7. Department of Traditional Pharmacy, School of Persian Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Medicinal Plants Published:2021


Abstract

Background: Arum rupicola Boiss. (Araceae Family) is used by the native people of southern areas of Iran as a soup called kardeh soup. Several flavonoids and phenol compounds have been identified from Arum species. Objective: The aim of this study was to evaluate antioxidant effect and total phenol contents as well as cytotoxic activity of the leaves of A. rupicola. Methods: Antioxidant activity of total methanol extract and fractions including n-hexane, chloroform, ethyl acetate and water residue were evaluated using FRAP and DPPH methods. Total phenol content was measured using Folin-Ciocalteu method. Cytotoxic activity of the extract and fractions were investigated against human breast cancer MCF-7, MDA-MB-231, and T47D cell lines by MTT assay. Further phytochemical isolation was done on the water residue using column chromatography. Results: According to the results, water residue showed the lowest IC50 value (186.7 μg/ml) and the total methanol extract showed the most antioxidant power (163.62 mmol FeSO4/100 g extract) and phenol content (135 μmol Gallic acid/g extract). The hexane fraction also showed the highest cytotoxic effect against MCF-7 breast cancer cell line with IC50 equal to 118.9 μg/ml. Phytochemical analysis of the water residue resulted in isolation and identification of three isoflavonoids named orobol, genistein and genistein 8-c-glucoside. Conclusion: Based on the identification of isoflavonoid compounds in this plant, its ability to be used as a phytoestrogenic supplement can be considered in future studies. © 2021 Institute of Medicinal Plants. All rights reserved.