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Medium-Based Optimization of an Organic Solvent-Tolerant Extracellular Lipase From the Isolated Halophilic Alkalibacillus Salilacus Publisher Pubmed



Samaeinouroozi A1 ; Rezaei S1 ; Khoshnevis N1 ; Doosti M2 ; Hajihoseini R1 ; Khoshayand MR3 ; Faramarzi MA1
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Authors Affiliations
  1. 1. Department of Pharmaceutical Biotechnology, Tehran University of Medical Sciences, P.O. Box 14155–6451, Tehran, 1417614411, Iran
  2. 2. Department of Medical Biochemistry, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Drug and Food Control, Tehran University of Medical Sciences, Tehran, 1417614411, Iran

Source: Extremophiles Published:2015


Abstract

A haloalkaliphilic solvent-tolerant lipase was produced from Alkalibacillus salilacus within 48 h of growth in liquid medium. An overall 4.9-fold enhanced production was achieved over unoptimized media after medium optimization by statistical approaches. Plackett–Burman screening suggested lipase production maximally influenced by olive oil, KH2PO4, NaCl, and glucose; and response surface methodology predicted the appropriate levels of each parameter. Produced lipase was highly active and stable over broad ranges of temperature (15–65 °C), pH (4.0–11.0), and NaCl concentration (0–30 %) showing excellent thermostable, pH-stable, and halophilic properties. The enzyme was optimally active at pH 8.0 and 40 °C. Majority of cations, except some like Co2+ and Al3+ were positive signals for lipase activity. In addition, the presence of chemical agents and organic solvents with different log Pow was well tolerated by the enzyme. Finally, efficacy of lipase-mediated esterification of various alcohols with oleic acid in organic solvents was studied. © 2015, Springer Japan.
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