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An Organic Solvent-Tolerant Lipase of Streptomyces Pratensis Mv1 With the Potential Application for Enzymatic Improvement of N6/N3 Ratio in Polyunsaturated Fatty Acids From Fenugreek Seed Oil Publisher



Vahidi M1, 2 ; Imanparast S1 ; Jahandar H2 ; Forootanfar H3 ; Mojtabavi S1 ; Faramarzi MA1
Authors
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Authors Affiliations
  1. 1. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155−6451, Tehran, 1417614411, Iran
  2. 2. Pharmaceutical Sciences Research Center, School of Pharmacy, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  3. 3. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran

Source: Journal of Food Science and Technology Published:2021


Abstract

Lipase-catalyzed esterification is an efficient technique in the production of polyunsaturated fatty acid (PUFA) concentrates which are applied for nutrition and health purposes. In this project, a solvent-tolerant lipase from Streptomyces pratensis MV1 was immobilized and purified by a hydrophobic support. The purified lipase revealed enhanced activity and stability towards chemicals, organic solvents, and a broad range of pH values. The production of lipase was enhanced to 7.0 U/mL after optimization by a central composite design. Acylglycerols (AGs) rich in α-linolenic acid (45%, w/w) were produced and a favorable n-6/n-3 free fatty acid (FFA) ratio of 1.1 was achieved in fenugreek seed oil using the immobilized lipase. The ability of S. pratensis lipase in ester synthesis and the improvement of n6/n3 FFA ratio make it a suitable candidate in food production industries. © 2020, Association of Food Scientists & Technologists (India).
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