Multiplex Pcr Analysis of 17 (11 Novels) Str Markers Linked to Six Autosomal Recessive Intellectual Disability Genes in Iranian Population Publisher Pubmed



Shirin G^{1, 2} ; Maryam A^{1, 2} ; Farideh ZR⁴ ; Javad TB² ; Sirous Z^{1, 3} Show Affiliations
Source: Clinical Laboratory Published:2016

Abstract

Background: Non-syndromic autosomal recessive intellectual disability (NS-ARID) is a heterogeneous neurodevel opmental disease. More research is needed to study the NS-ARID genes. Using STR markers linked to a specific gene, we can perform homozygosity mapping and prenatal diagnosis. One approach to investigate the NS-ARID genes in large families is homozygosity mapping. In this study, we surveyed allele frequency and allele heterozygosity of 17 NS-ARID STR markers linked to the six NS-ARID genes in Iranian population. Methods: The test group consisted of 120 unrelated healthy individuals. STR markers were designed using SERV software. Also, genotyping was done using multiplex PCR. Data was analyzed by Gene Mapper software. Allele frequency and observed heterozygosity rates were estimated using Power StatV12. Deviation from the Hardy Weinberg equilibrium (HWE) was performed based on the Exact test. Results: Out of 17 STR loci, 11 were novel. In total, 166 alleles were detected for the 17 markers. According to our study, the D9MAN1B1SD7.4, D19S872, D6GRIK2SI1.7, D19TECRSD1.8, D1ST3GAL3SI0.5, and D14ZC3H14SD5.3 STR loci were found to be the most informative and polymorphic STR markers for MAN1B1, ZNF526, GRIK2, TECR, ST3GAL3, and ZC3H14 genes, respectively. We also performed other statistical analyses on these STR markers and found that all of the 17 STRs were polymorphic and met the Hardy-Weinberg equilibrium. Conclusions: Finding novel STRs, with high allele heterozygosity, is one of the most significant outcomes of the present study. These findings can be useful for homozygosity mapping, PGD, and PND practices for the NSARID.