Separation and Quantification of Diazinon in Water Samples Using Liquid-Phase Microextraction-Based Effervescent Tablet-Assisted Switchable Solvent Method Coupled to Gas Chromatography-Flame Ionization Detection Publisher Pubmed



Kakaei H^{1, 2} ; Shahtaheri SJ^{3, 4} ; Abdi K⁵ ; Rahimi Kakavandi N¹ Show Affiliations
Source: Biomedical Chromatography Published:2023

Abstract

This study used a liquid-phase microextraction-based effervescent tablet-assisted switchable solvent method coupled to gas chromatography-flame ionization detection as an eco-efficient, convenient-to-use, cost-effective, sensitive, rapid, and efficient method for extracting, preconcentrating, and quantifying trace amounts of diazinon in river water samples. As a switchable solvent, triethylamine (TEA) was used. In situ generation of CO2 using effervescent tablet containing Na2CO3 and citric acid changed the hydrophobic TEA to the hydrophilic protonated triethylamine carbonate (P-TEA-C). CO2 removal from the specimen solution using NaOH caused P-TEA-C to be converted into TEA and led to phase separation, during which diazinon was extracted into the TEA phase. The salting-out process was helpful in enhancing extraction efficiency. In addition, a number of significant parameters that affect extraction recovery were examined. Under optimum conditions, the limit of detection and limit of quantitation were 0.06 and 0.2 ng/ml, respectively. The extraction recovery percentage and pre-concentration factor were obtained at 95 and 190%, respectively, and the precision (inter- and intra-day, relative standard deviation %, n = 5) was <5%. © 2023 John Wiley & Sons Ltd.