Enterolactone Reduces Telomerase Activity and the Level of Its Catalytic Subunit in Breast Cancer Cells

Enterolactone Reduces Telomerase Activity and the Level of Its Catalytic Subunit in Breast Cancer Cells Publisher

Ilbeigi D¹ ; Nourbakhsh M^{2, 3} ; Khaghani S¹ ; Einollahi N⁴ ; Kheiripour N¹ ; Gholinejad Z¹ ; Alaee M¹ ; Saberian M⁴

Show Affiliations

1. Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, P.O.Box: 1449614535, Tehran, Iran
3. Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular, Tehran University of Medical Sciences, P.O.Box: 1417614418, Tehran, Iran
4. Department of Clinical Laboratory Sciences, Tehran University of Medical Sciences, Faculty of Allied Medical Sciences, Tehran, Iran

Source: Cell Journal Published:2017

Abstract

Objective: There is a positive correlation between higher serum phytoestrogen concentrations and lower risk of breast cancer. The activation of telomerase is crucial for the growth of cancer cells; therefore, the aim of this study was to examine the effects of enterolactone (ENL) and enterodiol (END) on this enzyme. Materials and Methods: In this experimental study, we performed the viability assay to determine the effects of different concentrations of ENL and END on cell viability, and the effective concentrations of these two compounds on cell growth. We used western blot analysis to evaluate human telomerase reverse transcriptase catalytic subunit (hTERT) expression and polymerase chain reaction (PCR)-ELISA based on the telomeric repeat amplification protocol (TRAP) assay for telomerase activity. Results: Both ENL and END, at 100 uμM concentrations, significantly (P<0.05) reduced cell viability. However, only the 100 uμM concentration of ENL significantly (P<0.05) decreased hTERT protein levels and telomerase activity. Lower concentrations of ENL did not have any significant effects on telomerase activity and hTERT protein levels. Conclusion: High concentration of ENL decreased the viability of MCF-7 breast cancer cells and inhibited the expression and activity of telomerase in these cells. Although END could reduce breast cancer cell viability, it did not have any effect on telomerase expression and activity.

2. Microbiome and Breast Cancer: New Role for an Ancient Population, Frontiers in Oncology (2020)

Style	Citing Format
MLA	Ilbeigi D, et al.. "Enterolactone Reduces Telomerase Activity and the Level of Its Catalytic Subunit in Breast Cancer Cells." Cell Journal, vol. 19, no. , 2017, pp. 37-43.
APA	Ilbeigi D, Nourbakhsh M, Khaghani S, Einollahi N, Kheiripour N, Gholinejad Z, Alaee M, Saberian M (2017). Enterolactone Reduces Telomerase Activity and the Level of Its Catalytic Subunit in Breast Cancer Cells. Cell Journal, 19(), 37-43.
Chicago	Ilbeigi D, Nourbakhsh M, Khaghani S, Einollahi N, Kheiripour N, Gholinejad Z, Alaee M, Saberian M. "Enterolactone Reduces Telomerase Activity and the Level of Its Catalytic Subunit in Breast Cancer Cells." Cell Journal 19, no. (2017): 37-43.
Harvard	Ilbeigi D et al. (2017) 'Enterolactone Reduces Telomerase Activity and the Level of Its Catalytic Subunit in Breast Cancer Cells', Cell Journal, 19(), pp. 37-43.
Vancouver	Ilbeigi D, Nourbakhsh M, Khaghani S, Einollahi N, Kheiripour N, Gholinejad Z, et al.. Enterolactone Reduces Telomerase Activity and the Level of Its Catalytic Subunit in Breast Cancer Cells. Cell Journal. 2017;19():37-43.
BibTex	@article{ author = {Ilbeigi D and Nourbakhsh M and Khaghani S and Einollahi N and Kheiripour N and Gholinejad Z and Alaee M and Saberian M}, title = {Enterolactone Reduces Telomerase Activity and the Level of Its Catalytic Subunit in Breast Cancer Cells}, journal = {Cell Journal}, volume = {19}, number = {}, pages = {37-43}, year = {2017} }
RIS	TY - JOUR AU - Ilbeigi D AU - Nourbakhsh M AU - Khaghani S AU - Einollahi N AU - Kheiripour N AU - Gholinejad Z AU - Alaee M AU - Saberian M TI - Enterolactone Reduces Telomerase Activity and the Level of Its Catalytic Subunit in Breast Cancer Cells JO - Cell Journal VL - 19 IS - SP - 37 EP - 43 PY - 2017 ER -

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