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Anti-Invasion Activities of Heat-Killed Lactic Acid Bacteria Isolates Against Salmonella Enterica Serovar Typhimurium Publisher



Adzuan ASS1, 2 ; Mohamad SAS1, 2 ; Iberahim R3 ; Kamal NNSM2 ; Mutalib NA1 ; Hasbullah NI1, 3 ; Alsaydi M1, 4 ; Hasan N3 ; Oon LK5 ; Ajibola OO6 ; Alias R7 ; Mustakim M8 ; Rambely AS8 ; Mohamed E8 Show All Authors
Authors
  1. Adzuan ASS1, 2
  2. Mohamad SAS1, 2
  3. Iberahim R3
  4. Kamal NNSM2
  5. Mutalib NA1
  6. Hasbullah NI1, 3
  7. Alsaydi M1, 4
  8. Hasan N3
  9. Oon LK5
  10. Ajibola OO6
  11. Alias R7
  12. Mustakim M8
  13. Rambely AS8
  14. Mohamed E8
  15. Pourmand MR9
Show Affiliations
Authors Affiliations
  1. 1. Microbial Metabolite Laboratory, Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns), Universiti Teknologi MARA (UiTM), Selangor, Puncak Alam, Malaysia
  2. 2. School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), Selangor, Shah Alam, Malaysia
  3. 3. School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), Negeri Sembilan, Kuala Pilah, Malaysia
  4. 4. Department of Food Science and Technology, Faculty of Agriculture, Ibb University, Ibb, Yemen
  5. 5. Cell and Synthetic Biology Centre, Malaysia Genome and Vaccine Institute (MGVI), National Institutes of Biotechnology Malaysia (NIBM), Selangor, Kajang, Malaysia
  6. 6. Faculty of Resource Science and Technology, Universiti Malaysia Sarawak (UNIMAS), Sarawak, Kota Samarahan, Malaysia
  7. 7. Centre for Foundation and General Studies, Universiti Selangor, Selangor, Shah Alam, Malaysia
  8. 8. Centre for Medical Laboratory Technology Studies, Faculty of Health Sciences, Universiti Teknologi MARA (UiTM), Selangor, Puncak Alam, Malaysia
  9. 9. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Microbiology Published:2024


Abstract

Background and Objectives: The most common cause of severe foodborne salmonellosis is S. Typhimurium. Its interaction with intestinal epithelial cells is little known. Lactic acid bacteria (LAB) were recognized as a prominent probiotic gastroin-testinal microbiota of humans and animals that confer health-promoting and protective effects. This study aims to determine the anti-invasion and antibacterial effects of heat-killed LAB (HK-LAB) isolates against S. Typhimurium towards human intestinal cells. Materials and Methods: 12 HK-LAB isolates from 3 sources of origin (stingless bee, plant, and food) were tested to determine the adhesion of HK-LAB to Caco-2 cells, anti-invasion and antibacterial activities against S. Typhimurium, the adhesion and invasion pattern of S. Typhimurium on intestinal epithelial cells (Caco-2) and assessing the effect of LAB on the S. Typhimurium-host cell interaction. Results: Tairu isolates from food have the highest adhesion rate with 19 ± 1.32/10 Caco-2 cells followed by HK-LAB R-iso-late from plant 17 ± 0.70/10 Caco-2 cells, which is similar to the control (Lactobacillus casei). In the anti-invasion assay, the two HK-LAB isolates that had the strongest adherence to Caco-2 cells, Tairu-isolate inhibited at 78.1 ± 3.06% and R-isolate inhibited at 64.76 ± 9.02% compared to the positive control (63.81 ± 1.15%), which led to increased suppression of S. Ty-phimurium accordingly. Tairu and R isolates were tested for their antibacterial ability against S. Typhimurium. Both R and Tairu isolates displayed strong inhibition zones (27 ± 0.06 mm, 23 ± 0.06 mm) respectively. Conclusion: These findings suggest that the anti-invasion activities of HK-LAB R and Tairu may correlate to their bacteri-cidal effects that serve to protect the host from infection. © 2024 The Authors. Published by Tehran University of Medical Sciences.