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Lactobacillus Crispatus and Human Herpes Simplex Virus-2, an in Vitro Study Publisher



Goudarzi MM1 ; Fazeli MR2 ; Sepahi AA3 ; Eidi A4
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, Faculty of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
  2. 2. Department of Drug and Food Control, Pharmaceutical Quality Assurance Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Microbiology, Faculty of Biological Science, North of Tehran Branch, Islamic Azad University, Tehran, Iran
  4. 4. Department of Biology, Faculty of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

Source: Molecular Genetics# Microbiology and Virology Published:2023


Abstract

Abstract: Introduction: Genital infections have motivated researchers to explore the role of probiotics in the prevention and treatment of these infections. Aim: Development of a new potent and non-invasive way to prevent HSV-2 by probiotics. Methods: The anti-HSV-2 properties of culture cell-free supernatant (CFS) and cell body of Lactobacillus crispatus were evaluated by plaque assay method. The cytotoxicity and adhesion potential to HeLa cells were also evaluated. Finally, the composition of cell-free supernatant was characterized by chromatography-coupled mass spectrometry (GC-MS). Results: Assay showed a significant decrease in viability of HeLa cells upon exposure to a concentration of 108 CFU mL–1 as compared to 107 CFU mL–1 and other concentration of L. crispatus. Significant antiviral effects were recorded in the pre-treated Hela cells in both cell body form and CFS of L. crispatus. Similar results were obtained in the case of the L. crispatus ATCC 33820 strain. Carbohydrates (19.64%), unknown substances (18.89%), alcohols (14.79%), acids (11.90%), and peptides (11.89%) were the main ingredients of CFS. Conclusion: In this study, the potential of L. crispatus was assessed against HSV-2. L. crispatus significantly decreased infectivity of HSV-2. Coincidence or post-infection presence of L. crispatus showed almost no effect on HSV-2. These results support the hypothesis on the contribution of CFS of L. crispatus at initial virus-host interactions such as binding. Adhesion may be disturbed by lactobacilli through neutralizing virus particles. Both forms of L. crispatus could inhibit the virus even at neutral pH. © 2023, Allerton Press, Inc.